HOXA13对肾小管上皮细胞间充质转化和BMP-7表达的影响  

Effect of HOXA13 on epithelial-mesenchymal transition and BMP-7 expression in human renal tubular epithelial cells

作　　者：彭力[1] 何庆南[1] 李晓燕[1] 帅兰军[1] 陈海霞[1] 李永珍[1] 易著文[1] 

机构地区：[1]中南大学湘雅二医院小儿肾脏病研究室,湖南长沙410011

出　　处：《现代预防医学》2016年第10期1868-1872,1876,共6页Modern Preventive Medicine

摘　　要：目的探讨HOXA13对人血清白蛋白(HSA)诱导的人肾小管上皮细胞(HKC)间充质转分化(EMT)和骨形成蛋白7(BMP-7)表达的影响。方法体外培养人肾小管上皮细胞(HKC)经20 mg/ml人血清白蛋白作用48 h后,采用Real-Time PCR和免疫印迹法分别检测人肾小管上皮细胞角蛋白(CK)、波形蛋白(Vimentin)和HOXA13 mRNA和蛋白表达。应用脂质体转染技术上调人肾小管上皮细胞HOXA13表达,观察HOXA13不同表达水平对人血清白蛋白超载诱导CK、Vimentin、BMP-7表达改变的影响。结果 20 mg/ml HSA刺激HKC细胞48 h后,HKC出现EMT样改变:CK表达下降(P<0.01)、Vimentin表达增高(P<0.01);HSA超载同时使HKC细胞内HOXA13、BMP-7表达下降。基因转染上调HKC细胞内HOXA13表达后,能够显著抑制HSA超载诱导的EMT发生(P<0.05)和BMP-7表达下降(P<0.05)。结论 HOXA13拮抗白蛋白超载诱导肾小管上皮细胞发生EMT的作用可能与HOXA13上调BMP-7表达有关。Objective The aim of this study was to examine the effect of homeobox A13 （HOXA13） on epithelial - mesenchymal transition （EMT） and expressions of bone morphogenetic protein - 7 （ BMP - 7） induced by albumin - overload in human renal tubular epithelial cells （HKC）. Methods The cultured HKC cells were co - treated with 20mg/ml human serum albumin （HSA） for 48 hours. Protein and mRNA expressions of cytokeratin （CK）, vimentin and HOXA13 were assessed by western blot and real -time PCR, respectively. Meanwhile, protein and mRNA expressions of CK, vimentin, and BMP -7 were also detected in HKC cells transfected with lipofectamine contained HOXA13 DNA. Results HSA could induce EMT in HKC, suggested by decreased CK expression （P 〈 0. 01 ） and increased vimentin expression significantly （P 〈 0.01 ）. Up - regulated expression of HOXA13 transfected by lipofectamine could attenuate the level of EMT induced by HSA in HKC （P 〈 0.05）. Meanwhile, the decreased rate of BMP- 7 protein and mRNA expression induced by BSA were inhibited by overexpressed HOXA13 in HKC cells （P 〈 0. 05）. Conclusion Up -regulated expression of HOXA13 in HKC cells could inhibit the degree of EMT induced by albumin - overload, which involved BMP - 7 signaling pathway.

关 键 词：白蛋白超载 肾小管上皮细胞 上皮细胞-间充质转化 同源盒基因A13 骨形成蛋白-7 

分 类 号：R319[医药卫生—基础医学]