稻瘟病菌胁迫下水稻不同单基因系防卫相关基因表达分析  

Expression Analysis of Defense-related Genes in Different Rice(Oryza sativa) Monogenic Lines in Response to Magnaporthe oryzae Stress

作　　者：李伟[1] 王兴[1] 陈志雄[2] 程本义[3] 黄永相[1] 郭建夫[1] 杨仕华[3] 

机构地区：[1]广东海洋大学农学院,湛江524088 [2]华南农业大学农学院,广州510642 [3]中国水稻研究所,杭州310006

出　　处：《农业生物技术学报》2016年第6期781-789,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.31271688);中央级科研院所公益性科研业务费专项(No.2009RG005-3);广东省农业厅项目[粤农计(2011)143号]

摘　　要：水稻(Oryza sativa)不同稻瘟病抗性基因抗性水平存在显著差异,为了解防卫基因在不同抗性单基因系中的表达模式,本研究利用q RT-PCR方法比较了4个水稻抗稻瘟病单基因系IRBLKh-K3(Pik-h)、IRBLKs-F5(Pik-s)、IRBLta2-Re(Pita-2)和IRBLta-K1(Pita)中抗稻瘟病防卫相关基因几丁质酶基因1(chitinase 1,Cht-1)、β-1,3葡聚糖酶基因(β-1,3-glucanase,Glu)、苯丙氨酸解氨酶基因(phenylalanine ammonia lyase,PAL)和过氧化物酶基因(peroxidase,POX22.3)表达的差异。结果表明,在稻瘟病菌(Magnaporthe oryzae)胁迫后的6、12、24、36、48、72和96 h时间点,Cht-1和Glu的表达量在4个单基因系中的差异无明显的规律性。PAL在IRBLKh-K3(Pik-h)基因系中以及POX22.3在IRBLta2-Re(Pita-2)基因系中的表达量均持续高于在其余3个基因系中;前者的最高表达量至少是对照、IRBLta2-Re(Pita-2)、IRBLtaK1(Pita)和IRBLKs-F5(Pik-s)基因系的3.35、3.05、3.85和4.32倍,后者是对照、IRBLta-K1(Pita)、IRBLKhK3(Pik-h)和IRBLKs-F5(Pik-s)基因系的24.86、2.12、2.85和2.60倍。研究结果表明,PAL基因与POX22.3基因的表达分别受抗性基因Pik-h和Pita-2的特异调控参与抗性反应,为进一步研究抗性基因的抗性调控机制提供了依据。The resistant levels of rice （Oryza sativa） blast resistance genes are obviously different. Investigating the expression patterns of defense-related genes in different rice blast resistant monogenic lines is helpful for understanding the molecular mechanism of blast resistance gene against Magnaporthe oryzae. In the present study, the expression variation of defense-related genes chitinase 1 gene （Cht-1）, β-1,3-glucanase gene （Glu）, phenylalanine ammonia lyase gene （PAL） and peroxidase gene （POX22.3） were analyzed by qRT-PCR in 4 rice blast resistant monogenic lines, IRBLKh-K3 （Pik-h）, IRBLKs-F5 （Pik-s）, IRBLta2-Re （Pita-2） and IRBLta-K1 （Pita）. Results showed that the expression of Cht-1, Glu, PAL and POX22.3 in 4 monogenic lines were up-regulated at each or most stages after M. oryzae stress, but no consistent differences were observed about Cht-1 and Glu genes expression in 4 monogenic lines compared one another at 6, 12, 24, 36, 48, 72 and 96 h post-inoculation with M. oryzae except the peak expression of Cht-1 and Glu genes in IRBLta2-Re （Pita-2） in incompatible reaction and in IRBLKh-K3（Pik-h） in compatible reaction. However, both the expression levels of PAL in IRBLKh-K3（Pik-h） and POX22.3 in IRBLta2-Re （Pita-2） were lastly higher than that in the others in incompatible and compatible interaction with M. oryzae. In the incompatible interaction, the peak expression of PAL in IRBLKh-K3 （Pik-h） were 3.35, 3.05, 3.85 and 4.32 times of those in control, IRBLta2-Re （Pita-2）, IRBLta-K1 （Pita） and IRBLKs-F5 （Pik-s）, respectively. The POX22.3 expression in IRBLta2-Re （Pita-2） line increased more rapidly and significantly in higher levels within 96 h post-inoculation with incompatible M. oryzae isolates, which were 24.86, 2.12, 2.85 and 2.60 times over the expression in control, IRBLta-K1 （Pita）, IRBLKh-K3 （Pik-h） and IRBLKs-F5 （Pik-s） line at the time points of peak, respectively. In the compatible interaction, PAL expression level

关 键 词：水稻 稻瘟病菌 单基因系 防卫基因 抗性基因 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治]