大麦条纹病侵染对大麦叶片蛋白组的影响  

Effect on the Proteome of Barley(Hordeum vulgare) Leaves After Inoculation of Pyrenophora graminea

作　　者：孟亚雄[1,2] 何智宏[1,2] 汪军成[1,2] 司二静[1,2] 任盼荣 马小乐[1,2] 杨轲[1,2] 李葆春[1,3] 王化俊[1,2] 

机构地区：[1]甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质创新重点实验室,兰州730070 [2]甘肃农业大学农学院,兰州730070 [3]甘肃农业大学生命科学技术学院,兰州730070

出　　处：《农业生物技术学报》2016年第6期824-836,共13页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.31460347和No.31171558);国家大麦青稞产业技术体系(No.CARS-05)

摘　　要：大麦条纹病由麦类核腔菌(Pyrenophora graminea)引起,是一种世界性病害。为进一步探索条纹病与大麦(Hordeum vulgare)的相互作用,以大麦品种甘啤6号和Issto为实验材料,在接种麦类核腔菌(代号DWC)7和21 d后提取叶片蛋白,运用2-DE和质谱分析技术研究条纹病菌侵染后叶片蛋白质组学的变化。结果显示,与对照相比,甘啤6号和Isotta差异表达量在1.4倍以上的蛋白点28个,其中在甘啤6号中表达上调的蛋白点4个,下调的6个,诱导表达的2个,抑制表达的2个;在Isotta中,表达上调的蛋白点3个,下调的4个,诱导表达的4个,抑制表达的3个。质谱鉴定分析发现,表达上调的蛋白包括二磷酸核酮糖羧化酶A(2号蛋白点)、肌动蛋白(9号蛋白点)、核糖体再循环因子(ribosome-recycling factor,RRF)(10号蛋白点)、ATP合酶γ链(11和27号蛋白点)、琥珀酸脱氢酶(辅酶q)黄素蛋白亚基(15号蛋白点)和假定蛋白(26号蛋白点);表达下调的包括过氧化物还原蛋白(4号蛋白点)、1,5-二磷酸核酮糖羧化酶大亚基(ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit,Ru Bis Co)(3、5、12、14、16和18号蛋白点)、ATP合酶CF1α亚基(13号蛋白点)、Ycf3蛋白(17号蛋白点)和α-1,4葡聚糖蛋白合酶(alpha-1,4-glucanprotein synthase,UTPG)(28号蛋白点);诱导表达蛋白包括假定蛋白(6号蛋白点)、脂氧合酶2(lipoxygenase2,LOX2)(7号蛋白点)、捕光叶绿素a/b结合蛋白质(light harvesting chlorophyll a/b-binding protein,LHCP)(19号蛋白点)、3-磷酸甘油酸激酶(3-phosphoglycerate kinase,PGK)(20号蛋白点)、凝集素(21号蛋白点)和ATP合酶γ链(22号蛋白点);抑制表达的蛋白包括Ru Bis Co(1、8、24和25号蛋白点)和二磷酸核酮糖羧化酶小链(ribulose bisphosphate carboxylase small chain,Ru BPCase)(23号蛋白点)等。按照其功能分类,这些差异表达蛋白点分别参与了光合作用、蛋白质生物合成、植物防卫反应、能量代谢和细胞信号转导、Barley stripe disease is caused by Pyrenophora graminea, and is a worldwide disease. To explore the interaction mechanism between Hordeum vulgare and Pyrenophora graminea, total protein of Ganpi 6 and Isotta leaves which were inoculated with Pyrenophora graminea （DWC） for 7 and 21 days were extracted to analyze proteomic changes. Twenty eight protein spots were found which showed a change of more than 1.4-fold between the inoculated and the mock-inoculated samples of Ganpi 6 and Isotta, of which 4 were up-regulated, 6 were down-regulated, 2 were induced and 2 were inhibited for Ganpi 6, and 3 were up-regulated, 4 were down-regulated, 4 were induced and 3 were inhibited for Isotta. The up-regulated proteins included ribulose-bisphosphate carboxylase activase A （No. 2 spot）, actin （No. 9 spot）, ribosome-recycling factor （RRF）（No. 10 spot）, ATP synthase gamma chain（No. 11 and 27 spots）, succinate dehydrogenase [ubiquinone] flavoprotein subunit （No. 15 spot） and predicted protein （No. 26 spot）. The down-regulate protein spots included bas1 protein （No. 4 spot）, ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit （RuBisCo）（No. 3, 5, 12, 14, 16 and 18 spots）, ATP synthase CF1 alpha subunit （No. 13 spot）, Ycf3 （No. 17 spot） and alpha-1,4-glucan-protein synthase （UTPG）（No. 28 spot）. The induced expression protein spots included predicted protein （No. 6 spot）, lipoxygenase 2 （LOX2）（No. 7 spot）, light harvesting chlorophyll a/b-binding protein （LHCP）（No. 19 spot）, 3-phosphoglycerate kinase （PGK）（No. 20 spot）, lectin （No. 21 spot） and ATP synthase gamma chain （No. 22 spot）. The inhibited protein spots included RuBisCo （No. 1, 8, 24, 25 spots） and ribulose bisphosphate carboxylase small chain （RuBPCase）（No. 23 spot）. These protein spots were identified by MALDI -TOF/ TOF MS and protein database searching by MASCOT program. The functions of the 28 protein were related to photosynthesis, protein synthesis, plan

关 键 词：蛋白质组学 大麦 大麦条纹病 叶片蛋白 双向电泳 

分 类 号：S18[农业科学—农业基础科学]