来源于中国南海的微生物新种乙醇速生杆菌(Celeribacter ethanolicus)多相分类研究（英文）  

Celeribacter ethanolicus sp. nov., isolated from seawater of the South China Sea

作　　者：简书令 吴月红[1] 买热帕提阿依 迪丽拜尔托乎提 Aharon Oren 许学伟[1] 

机构地区：[1]国家海洋局第二海洋研究所国家海洋局海洋生态系统与生物地球化学重点实验室,浙江杭州310012 [2]新疆师范大学生命科学学院,新疆乌鲁木齐830054 [3]Department of Plant and Environmental Sciences,The Alexander Silberman Institute of Life Sciences,The Hebrew University of Jerusalem,The Edmond J.Safra Campus,Givat Ram,Jerusalem 91904,Israel

出　　处：《微生物学通报》2016年第5期907-916,共10页Microbiology China

基　　金：National Basic Research Program of China(No.2014CB441503);National Natural Science Foundation of China(No.41406174);Natural Science Foundation of Zhejiang Province(No.LY14D060006)~~

摘　　要：【目的】研究来源于南海海水的一株速生杆菌属菌株NH195T的多相分类。【方法】采用表型、基因型和化学分类方法,并综合系统发育关系结果,分析菌株NH195T的分类学地位。【结果】菌株NH195T是一株革兰氏阴性、好氧、杆状、无运动性细菌;能积累Poly-β-hydroxy butyrate(PHB);能在0.5%-10.0%(质量体积比)NaCl浓度,pH 5.0-9.0和20-40°C条件下生长,最适NaCl生长浓度为1.0%-3.0%;氧化酶、触酶和脲酶反应结果阳性。菌株NH195T主要呼吸醌为Q-10,主要脂肪酸为C18:1ω7c、C18:1ω6c和11 methyl C_(18:1)ω7c,主要极性脂为磷脂酰胆碱、磷脂酰甘油、双磷脂酰甘油、一个未知的氨基脂和两个未知脂。基因组G+C含量为61.3 mol%。基于16S rRNA基因的系统发育结果显示,菌株NH195T隶属于速生杆菌属;其与速生杆菌属标准菌株的16S rRNA基因相似性范围为94.4%-97.7%。菌株NH195T与速生杆菌属标准菌株C.halophilus ZXM137T和C.indicus P73T的平均核苷酸一致性(ANI)分别为78.6%和78.0%;基于基因组数据计算所得的DNA杂交同源率分别为26.1%和23.0%。【结论】基于表型和基因型结果,菌株NH195T代表了速生杆菌属一个新物种,命名为Celeribacter ethanolicus,标准菌株为NH195T(CGMCC 1.15406T=JCM 31095T)。[Objective] A novel Celeribacter strain NH195x was isolated from seawater of the South China Sea and was subjected to polyphasic taxonomic study. [Methods] Phenotypic, genotypic and chemotaxonomic characterizations, as well as phylogenetic inference were performed in this study. [Results] Strain NH195x was a Gram-stain-negative, aerobic, rod-shaped, non-motile bacterium that accumulates poly-fl-hydroxybutyrate （PHB）. The isolate grew in media containing 0.5%-10.0% （W/F） NaC1 （optimally at 1.0%-3.0%） at 20-40℃ and pH 5.0-9.0, and was positive for oxidase, catalase and urease. The respiratory quinone was Q-10. The major fatty acids （〉10%） were C18：1co7c, C18：1co6c and 11 methyl C18：1co7c. The major polar lipids were phosphatidylcholine （PC）, phosphatidylglycerol （PG）, diphosphatidylglycerol （DPG）, one unidentified aminolipid and two unidentified lipids. The genomic DNA G＋C content was 61.3 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain NH195T could be assigned to the genus Celeribacter. The 16S rRNA gene sequence similarities between the isolate and the type strains of Celeribacter species with validly published names were in the range 94.4%-97.7%. Strain NH195T exhibited average nucleotide identity （ANI） values of 78.6% and 78.0% to C. halophilus ZXM137T and C. indicus P73T respectively. The genome-to-genome distance analysis revealed that strain NH195T shared 26.1% DNA relatedness with C. halophilus ZXM137T and 23.0% with C. indicus P73T, respectively. [Conclusion] On the basis of phenotypic and genotypic characteristics, strain NH195T represents a novel species within the genus Celeribacter, for which the name Celeribacter ethanolicus is proposed. The type strain is NH 195T （=CGMCC 1.15406T=JCM 31095T）.

关 键 词：乙醇速生杆菌 多相分类 海水 中国南海 

分 类 号：Q93[生物学—微生物学]