我国十省份羊巴贝斯虫的分子流行病学调查  

Molecular epidemiological investigation of ovine Babesia spp. in 10 provinces of China

作　　者：杨强[1,2] 刘爱红[1] 刘军龙[1] 杨吉飞[1] 李有全[1] 刘志杰[1] 殷宏[1] 罗建勋[1] 马润林[2] 关贵全[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046 [2]中国科学院遗传与发育生物学研究所分子发育生物学国家重点实验室,北京100101

出　　处：《中国兽医科学》2016年第5期597-601,共5页Chinese Veterinary Science

基　　金：国家重点基础研究发展计划(973)项目(2015CB150300);国家自然科学基金资助项目(31372432;3402189);农业科技创新工程(ASTIP)

摘　　要：为了调查和分析羊的巴贝斯虫(莫氏巴贝斯虫和羊巴贝斯虫未定种)在我国的分布情况,2010—2014年间,于我国10个省份共采集到823份绵羊和山羊血液样本,应用区分莫氏巴贝斯虫和羊巴贝斯虫未定种的实时荧光定量PCR方法,对提取的基因组DNA进行了检测。结果显示,除了海南省,其他省份都检测到莫氏巴贝斯虫的感染,总体阳性率为5.95%(49/823),其中河南省的阳性率最高,为10.47%(9/86);而羊巴贝斯虫未定种只在湖北、贵州和甘肃省检测到,阳性率分别为4.55%(2/44)、6.38%(3/47)和0.62%(2/322)。本研究首次应用实时荧光定量PCR对我国羊巴贝斯虫病的流行状况进行大范围的调查,为进一步了解我国羊巴贝斯虫的分布情况和制定羊巴贝斯虫病防控策略提供了可靠的分子流行病学信息。To investigate and analyze ovine Babesia （Babesia motasi and Babesia sp. ） distribution in China,823 sheep and goat blood samples from 10 provinces were collected during 2010--2014. The ge- nomic DNAs were extracted from these blood samples. A real-time PCR method that can differentiate B. mo- tasi and Babesia sp. was used to detect the infection of ovine Babesia. Results showed that except Hainan Province,B. motasi was detected from the other 9 provinces. The highest positive rate was pre- sent in the samples from Henan Provinee,10.47%（9/86）.The total positive rate was 5.95%（49/823）. Babesia sp.,however,was lust detected from Hubei,Guizhou and Gansu provinces. The positive rate was 4.55%（2/44）,6.38%（3/47） and 0.62%（2/322）,respectively. This is the first large-scale investiga-tion for ovine babesiosis using real-time PCR in China. lt provides the reliable molecular epidemiolo- gical information for further understanding the distribution of ovine Babesia and developing preven- tion and control strategies of the diseases in this country.

关 键 词：羊巴贝斯虫 流行病学调查 实时荧光定量PCR 

分 类 号：S852.723[农业科学—基础兽医学]