光合产电微生物群落构建及光照培养对其产电性能的影响  

Construction of an electrogenic microbial community including phototrophic bacterial and effect of illumination on its electrogenic activity

作　　者：郑伟[1] 黄满红[1] 陈东辉[1] 陈亮[1] 

机构地区：[1]东华大学环境科学与工程学院国家环境保护纺织污染防治工程技术中心,上海201620

出　　处：《环境工程学报》2016年第5期2719-2724,共6页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(21477018)

摘　　要：采用淡水沉积物为接种来源,培养出光合产电微生物群落。将其与藻阴极联用组建了完整的光合作用微生物燃料电池时,功率密度达到(157.5±3.1)m W/m2。采用循环伏安法及电化学阻抗谱对该群落的电化学性能进行了测试。PCR-DGGE及紫外可见吸收光谱分析显示,该群落含有Ectothiorhodospiraceae科及Chloroflexi门不产氧光合细菌、产电菌Arcobacter butzleri、发酵细菌及其他细菌。对该群落进行长期黑暗培养或长期光照培养时,其产电性能均得到了提高,但功率密度测试显示,光照培养微生物燃料电池最大功率密度为(180.1±8.7)m W/m2,高于黑暗培养的微生物燃料电池(160.7±11.4)m W/m2。电化学测试也显示,光照培养的阳极产电性能优于黑暗培养的阳极。A natural electrogenic microbial community, which included anoxygenic phototrophic bacteria, was isolated from a freshwater deposit. When this community was used in a photosynthetic microbial fuel cell （MFC） with an algal cathode, the maximum power density achieved was （157.5± 3. 1 ）mW/m2. Electro- chemical performance of the anode was evaluated by cyclic voltammetry and electrochemical impedance spectros- copy tests. PCR-DGGE and UV-vis analysis indicated the existence of Ectothiorhodospiraceae, Chloroflexi, Arco- bacter butzleri, fermentative bacteria, and other bacteria in the microbial community. Electrochemical perform- ance of the microbial community improved by long-term operation, both with and without illumination; the maxi- mum power densities of the MFC with and without illumination were （ 180.1± 8.7） mW/m2and （ 160.7 ± 11.4） mW/m2, respectively. In addition, the electrochemical tests confirmed that the performance of the anode culti- vated under illumination was better than that of the anode cultivated in the dark.

关 键 词：产电微生物群落 光照培养 产电性能 光合细菌 

分 类 号：X382[环境科学与工程—环境工程]