温度驯化对斑马鱼酶活性的影响  

The Effect of Temperature Acclimation on Enzyme Activity of Zebra Fish

作　　者：雷瑾[1] 吕耀平[1] 傅旭豪 郑燕倩[1] 

机构地区：[1]丽水学院生态学院,浙江丽水323000

出　　处：《丽水学院学报》2016年第2期55-59,共5页Journal of Lishui University

基　　金：浙江省大学生科技创新活动计划暨新苗人才计划项目(2015R431013)

摘　　要：实验研究在15、20、25和30℃驯化下斑马鱼酸性磷酸酶(ACP)、乳酸脱氢酶(LDH)和过氧化氢酶(CAT)活性的改变,结果表明不同酶对温度变化的响应机制存在差异。在驯化第15 d,斑马鱼的ACP性随温度降低而显著上升,第20和25 d 15℃组的ACP活性仍显著高于20、25和30℃组。低温同样诱导斑马鱼LDH活性的增加,但不同温度组之间的差异不如ACP明显。相比ACP和LDH,温度对斑马鱼CAT活性的影响较小。虽然在第20 d 15℃组的CAT活性显著高于20、25和30℃组,但在第0、10、15和25 d任意两个温度组的CAT活性无显著差异。This study investigated the effect of different temperature acclimation （15, 20, 25 and 30℃） on the acid phosphatase （ACP）, lactate dehydrogenase （LDH） and catalase （CAT） activities of zebra fish. The results showed that there were differences in the response mechanism of different enzymes to temperature changes. In the fifteenth day, ACP activity of zebra fish significantly increased with the decrease of temperature. In the twentieth and twenty-fifth day, ACP activity of zebra fish of 15℃ group was still higher than those of 20, 25 and 30℃ group. Although LDH activity of zebra fish was increased under low temperature environment, the difference was not obvious as ACP between different temperature groups. Compared with ACP and LDH, temperature has little effect on the CAT activity of zebra fish. Although CAT activity of zebra fish of 15℃ group was higher than those of 20, 25 and 30℃ group at the twentieth day of acclimation, there was no significant difference between any two temperature groups at the zero, fifth, tenth, fifteenth and twenty-fifth days of acclimation.

关 键 词：温度 斑马鱼 酸性磷酸酶 乳酸脱氢酶 过氧化氢酶 

分 类 号：S917[农业科学—水产科学]