石斛多糖的提取分离及其抗氧化性能的研究  

Study on the Extraction,Separation and Antioxidation Ability of Polysaccharides from Dendrobium Nobile

作　　者：刘坚[1] 饶赛男 豆毛 熊丽萍[1] 

机构地区：[1]华东交通大学理学院,江西南昌330013

出　　处：《江西化工》2016年第2期73-75,共3页Jiangxi Chemical Industry

基　　金：江西省教育厅科技项目(GJJ150484);华东交通大学校立科研课题项目(15LX01)

摘　　要：本文运用正交实验设计L9（3^4）对水煎提取石斛中石斛多糖的工艺进行优选,结果表明溶剂用量和提取次数对多糖提取含量的影响最大,提取温度和时间差异无显著性,采用短时多次提取工艺更加省时合理。选择铁皮石斛,利用化学发光法分别在羟基自由基、超氧阴离子体系中进行了研究。结果显示在不同的自由基体系下铁皮石斛多糖的作用不同,有清除作用,也有促进氧化作用。The optimum preparation procedure for the Polysaccharide contained in Dendrobium nobile with water - extracting were studied by L9 （3^4） orthngonal experimental design,the result showed that water-adding amount and extracting times has the largest influences on the index, and to the result of decocting time temperature was indistinct. The process with short - time and extracting many times was much more effective. To research the relation among polysaccharide content and the antioxygenation ability which is measured by chemluminescence technology. The result shows that it has distinct effect in the different system, and the grads relation between effect and consistence of homogeneity polysaccharide in the same system.

关 键 词：石斛多糖 正交实验 抗氧化 化学发光法 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学]