地塞米松对异丙肾上腺素所致小鼠急性心肌损伤及脾脏T细胞亚群的影响  

Effects of Dexamethasone on Acute Myocardial Injury induced by Isoproterenol and T Cells in Spleen

作　　者：张严高[1] 余磊[2] 王建莉[2] 朱闽湘[3] 尹戴佳佳 

机构地区：[1]南京军区杭州疗养院疗养一科,杭州310007 [2]浙江大学免疫研究所,杭州310058 [3]中国人民解放军第117医院神经外科,杭州310013

出　　处：《微循环学杂志》2016年第2期9-13,79,共5页Chinese Journal of Microcirculation

摘　　要：目的:观察地塞米松(DEX)对异丙肾上腺素(ISO)致小鼠急性心肌缺血损伤的影响,并探讨其作用机制。方法:16只昆明种小鼠随机分成正常对照组(CON组)、ISO组、DEX预处理组(DEX+ISO组)、DEX后处理组(ISO+DEX组),每组各4例。ISO组腹腔注射ISO(5mg/kg),1次/天,连续3天;DEX+ISO组于ISO注射前30min腹腔注射DEX(1.25mg/kg),连续3天;ISO+DEX组于实验第2天和第3天腹腔注射ISO后30min,再腹腔注射DEX(1.25mg/kg);CON组腹腔注射等量生理盐水,连续3天。实验第4天,各组小鼠心脏采血检测血清谷草转氨酶(AST)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同功酶(CK-MB);摘取心脏进行HE染色,观察各组心肌组织形态学改变;摘取脾脏,流式细胞术检测脾组织匀浆T细胞亚群分布。结果:(1)与CON组比较,ISO组AST、LDH、CK均显著升高(P<0.01);心肌组织损害明显加重(P<0.01);脾组织CD8^+、CD4^+CD69^+T细胞表达上升(P<0.05,P<0.01)。(2)与ISO组比较,DEX+ISO组AST、CK、LDH、CK-MB均下降(P<0.05或P<0.01);心肌组织损害明显改善(P<0.01);脾组织CD4^+、CD8^+T细胞表达显著下降(P<0.01),CD4^+CD69^+T细胞表达显著上升(P<0.01)。(3)与ISO组比较,ISO+DEX组CD8^+T细胞表达下降(P<0.05),其余均无明显变化(P>0.05)。结论:DEX预处理可以明显改善ISO致小鼠急性心肌缺血损伤,其作用机制可能与DEX介导的T细胞亚群免疫应答功能改变有关。Objective：To investigate potential protective effect of glucocorticoid receptor （GR）agonist dexam‐ethasone（DEX） on isoproterenol（ISO）‐induced myocardial injury and the possible mechanism .Method：The sixteen mice were randomly divided into control （CON） group（n= 4） ,ISO group（n= 4） ,dexamethasone pretreatment （DEX＋ISO） group （n=4）and dexamethasone treatment（ISO＋DEX） group（n=4） .ISO group ,DEX＋ ISO group and ISO＋DEX group received 5mg/kg isoproterenol hydrochloride ,daily intraperitoneal injection of 1 time ,contin‐uous 3 days .DEX＋ISO group given dexamethasone（1 .25mg/kg）injection by intraperitoneal injection as a pretreat‐ment before treatment 30 minutes .At the 2nd and 3rd day ,dexamethasone（1 .25mg/kg） was given after ISO injec‐ted 30 minutes in ISO＋DEX group .The serum AST ,LDH ,CK ,and CK‐MB were detected .Myocardial tissue in‐jury was assessed by HE staining .Flowcytometry was adopted to detect the expression of T cell subpopulation .Re‐sults：①Compared with control group ,the levels of AST ,LDH and CK were significantly increased ;the degree of myocardial injury were greatly deteriorated in ISO group （P〈 0 .01）;the expression 0f CD8＋ T cells and CD4＋CD69＋ T cells were greatly increased（P〈0 .05 ,P〈0 .01） .②Compared with ISO group ,the levels of AST and CK was significantly reduced（P〈0 .01）;the levels of LDH and CK‐MB were reduced（P〈0 .05）;the degree of myocar‐dial injury was greatly improved（P〈0 .01） in DEX＋ ISO group;the expression of CD4＋ T cells and CD8＋ T cells were significantly reduced（P〈0 .01） ,the expression of CD4＋ CD69＋ T cells were significantly increased（P〈0 .01） in DEX＋ISO group .③Compared with ISO group ,the expression of CD8＋ T cells were reduced（P〈0 .05） and the other wasn＇t difference （P〈0 .05） in ISO＋DEX group .Conclusion：DEX pretreatment has protective effect against ISO‐induced myocardial injury .The mechanism might

关 键 词：地塞米松 异丙肾上腺素 心肌损伤 CD4+ CD8+ CD69+ 小鼠 

分 类 号：R542.2[医药卫生—心血管疾病]