草鱼PRL基因多态性与幼鱼生长性状和肌肉成分的关联分析  

Polymorphisms of the PRL gene and their associations with growth traits and flesh components in juvenile grass carp, Ctenopharyngodon idella

作　　者：傅建军[1,2] 张猛[1] 沈玉帮[1] 陈勇 徐晓雁[1] 李家乐[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306 [2]中国水产科学研究院淡水渔业研究中心,农业部淡水渔业与种质资源利用重点实验室,江苏无锡214081 [3]通威股份有限公司水产工程技术研究中心,四川成都610093

出　　处：《中国水产科学》2016年第3期491-499,共9页Journal of Fishery Sciences of China

基　　金：现代农业产业技术体系建设项目(CARS-46-04);上海市种业发展项目(2012NY10);通威股份有限公司产学研项目(TW2014F003);上海高校水产学一流学科建设项目

摘　　要：为了探索草鱼(Ctenopharyngodon idella)PRL(prolactin)基因多态性与草鱼生长性状和肌肉成分的关联性,通过测序法从10尾草鱼的PRL基因中筛选到6个突变率高于30%的变异位点,包括5个单核苷酸变异位点和1个插入型突变(–/CACTCACTA),分别命名为2551G>A、2639G>C、3247A>G、5197T>G、5897G>A和3391–>+。利用AS-PCR(allele-specific PCR)和基因分型技术对192尾4月龄草鱼的PRL基因变异位点进行检测;基于一般线性模型对变异位点多态性与草鱼生长性状和肌肉成分进行相关性分析。研究发现,2639G>C、3391–>+和5197T>G对体长和体重具有显著影响(P<0.05),2639G>C对肌肉粗蛋白含量具有显著影响(P<0.05)。单个位点基因型比较发现,3391–>+的突变型(–+和++)个体的体长和体重均显著高于野生型(––)个体(P<0.05);2639G>C的GC及5197T>G的突变型(TG和GG)个体的体长和体重分别显著低于2639G>C的GG和5197T>G的TT野生型(P<0.05);2639G>C突变型(CC)个体的粗蛋白含量显著高于2639G>C其他基因型(GG和GC)个体(P<0.05)。两位点组合比较发现,含3391–>+突变型(–+和++)的组合对应体长和体重普遍高于其他组,含2639G>C的CC突变型的组合对应粗脂肪含量和粗蛋白含量普遍较高,其中粗蛋白含量显著高于其他组(P<0.05)。研究表明,草鱼PRL基因多态性与草鱼生长性状和肌肉成分间存在显著关联,推测相关变异位点可作为草鱼生长和肉质改良的候选辅助标记。We analyzed the associations between the polymorphisms of the PRL （prolactin） gene and the growth traits and flesh components of the grass carp, Ctenopharyngodon idella. Six variable sites with mutation rates exceeding 30% were detected in 10 individuals by sequencing the full length of the PRL gene. These included five single-nucleotide polymorphic loci and one insert mutation （-/CACTCACTA）, designated 2551G〉A, 2639G/C, 3247A〉G, 5197T〉G, 5897G〉A, and 3391-/＋, respectively. The polymorphisms at the variable sites were examined in 192 juvenile C. idella using allele-specific PCR and genotyping. The associations between the polymorphisms at these loci and the growth traits and flesh components of the fish were analyzed with general linear models. The polymorphisms at three loci （2639G〉C, 3391-〉＋, and 5197T〉G） and one locus （2639G〉C） were significantly associated with growth traits and the flesh protein content （P〈0.05）, respectively. Multiple comparisons of the traits were made with the genotypes at each locus. The standard length and bodyweight of individuals with the mutant genotypes （-＋ and ＋＋） at 3391-〉 ＋ were significantly higher than those of individuals with the wild-type genotype （- -） （P 〈 0.05）. In contrast, the standard length and bodyweight of individuals with the mutant genotypes at 2639G〉C （GC） and 5197T〉G （TG and GG） were significantly lower than those of individuals with the wild-type genotypes at 2639G〉C （GG） and 5197T〉G （TT） （P〈0.05）. For the, Individuals with the mutant genotype CC at 2639G〉C had significantly higher flesh protein content than individuals with the other genotypes （GG and GC） （P〈0.05）. Similar results were found in multiple comparisons for the traits of two loci groups. The standard length and bodyweight of individuals that contained mutant genotypes （-＋ and ＋＋） at 3391-〉＋ were generally higher than those of individuals with the wild-type genotype （- -）. Th

关 键 词：草鱼 PRL基因 多态性 生长性状 肌肉成分 相关性 

分 类 号：S917[农业科学—水产科学]