HPLC-UV-ELSD同时测定复方红景天胶囊中8种成分  

Simultaneous determination of eight constituents in Compound Rhodiola Capsule by HPLC-UV-ELSD

作　　者：殷洪梅[1,2] 刘俊超[1,2] 徐忠坤[1,2] 韦迎春[1,2] 陶永华[1,2] 付娟[1,2] 孙永城[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中草药》2016年第5期771-774,共4页Chinese Traditional and Herbal Drugs

基　　金：科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203);负责人:王振中

摘　　要：目的采用HPLC-UV-ELSD同时测定复方红景天胶囊中大花红天素、没食子酸、红景天苷、酪醇、哈巴苷、哈巴俄苷、安格洛苷C、肉桂酸8种成分。方法应用Kromasi L色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.3%冰醋酸水溶液,梯度洗脱,体积流量1.0 m L/min,紫外检测波长275 nm,柱温30℃,蒸发光散射检测器漂移管温度40℃,载气N2,压力150 k Pa。结果大花红天素、没食子酸、红景天苷、酪醇、哈巴苷、哈巴俄苷、安格洛苷C、肉桂酸8种成分能够达到很好分离;其线性范围分别为67.084~670.84(R^2=0.999 2)、11.410~114.100(R^2=0.999 4)、78.995~789.95(R^2=0.999 6)、19.625~196.25(R^2=0.999 7)、59.368~593.68(R^2=0.999 8)、62.585~625.85(R^2=0.999 5)、55.045~550.45(R^2=0.999 6)、6.895~68.95μg/m L(R^2=0.999 8);平均回收率分别为100.8%、98.9%、100.1%、100.8%、98.9%、99.6%、100.7%、99.2%,RSD分别为0.64%、0.56%、0.35%、0.65%、0.26%、0.58%、1.00%、0.64%(n=6)。结论该方法分析简便、准确,可作为该制剂的定量分析方法。Objective To develop an HPLC-UV-ELSD method for the simultaneous determination of crenelatin, gallic acid, salidroside, tyrosol, harpagide, harpagoside, angoroside C, and cinnamic acid in Compound Rhodiola Capsule. Methods Kromasil Cl8 column （250 mm×4.6 mm, 5 um） was adopted. The mobile phase was composed of acetonitrile （A） and 0.3% HAC （B） with gradient elution. The flow rate was 1.0 mL/min and the detection wavelength was 275 nm. the column temperature was 30 ℃, and the evaporative light-scattering detector （ELSD） drift tube temperature was 40 ℃, and gas pressure of 1.5 bar （150 kPa）. Results Crenelatin, gallic acid, salidroside, tyrosol, harpagide, harpagoside, angoroside C, and cinnamic acid were separated well. The linear calibration curves were obtained in 67.084--670.84 ug/mL for crenelatin, R2 = 0.999 2; 11.410--114.100 ug/mL for gallic acid, R2 = 0.999 4; 78.995--789.95ug/mL for salidroside, R2 = 0.999 6; 19.625--196.25 ug/mL for tyrosol, R2 = 0.999 7; 59.368--593.68ug/mL for harpagide, R2 = 0.999 8; 62.585--625.85ug/mL for harpagoside, R2 = 0.999 5; 55.045--550A5ug/mL for angoroside C, R2 = 0.999 6; and 6.895--68.95ug/mL for cinnamic acid, R2 = 0.999 8. The average recoveries of the eight constituents were 100.8%, 98.9%, 100.1%, 100.8%, 98.9%, 99.6%, 100.7%, and 99.2% with RSD of 0.64%, 0.56%, 0.35%, 0.65%, 0.26%, 0.58%, 1.00%, and 0.64%. Conclusion The method is convenient, accurate, and can be used for the simultaneous determination of the preparation.

关 键 词：复方红景天胶囊 HPLC-UV-ELSD 大花红天素 没食子酸 红景天苷 酪醇 哈巴苷 哈巴俄苷 安格洛苷C 肉桂酸 

分 类 号：R286.02[医药卫生—中药学]