六味地黄颗粒干预下的哮喘大鼠模型肺组织中TGF-β1的变化  

The changes of transforming growth factor beta1 expression in lung tissues of rat asthmatic models after administration with traditional Chinese medicine Liuwei Dihuang Granule

作　　者：陈晶[1] 宋曦[1] 黄小琪[1] 

机构地区：[1]广西中医药大学第一附属医院医学分子生物学实验室,广西南宁530023

出　　处：《右江民族医学院学报》2016年第2期139-142,共4页Journal of Youjiang Medical University for Nationalities

基　　金：广西科学基金项目(2013GXNSFBA019136)

摘　　要：目的观察六味地黄颗粒干预下哮喘大鼠肺组织中TGF-β1的变化,为研究中医药防治小儿哮喘提供实验依据。方法以卵清白蛋白建立哮喘大鼠模型并分组,通过HE染色观察大鼠肺组织变化;免疫组织化学法检测各组肺组织TGF-β1蛋白表达量,并用ELISA法检测各组大鼠肺泡灌洗液TGF-β1含量。结果 HE染色显示哮喘模型对照组肺组织炎症细胞浸润,支气管黏膜皱襞增多,气道平滑肌明显增厚。免疫组化结果显示,与空白对照组相比,哮喘模型对照组TGF-β1蛋白表达明显升高。与哮喘模型对照组相比,六味地黄颗粒组和阳性对照地塞米松组TGF-β1均明显降低,且六味地黄颗粒组的TGF-β1水平与空白对照组水平相比,差异无统计学意义。ELISA检测结果显示,各组大鼠肺泡灌洗液中TGF-β1含量变化与免疫组化结果一致。结论在六味地黄颗粒干预下,哮喘大鼠模型肺组织TGF-β1的表达量下调。Objective To study the effects of traditional Chinese medicine Liuwei Dihuang Granule on transforming growth factor beta1 expression in lung tissues of rat asthmatic models and to provide experimental evidence for Chinese medicine in prevention and treatment of child asthma. Methods We built rat asthmatic models by using OVA and the rats were grouped. Then an immunohistochemistry was used to detect the pro- tein expression of transforming growth factor betal in lung tissues and an ELISA method was used to measure the quantity of transforming growth factor betal in bronchoalveolar lavage fluid （BALF）. Results The re- suits of HE staining presented obviously inflammatory cell infiltration of lung tissues, bronchial mucosal folds increasing and airway smooth muscles thickening in asthmatic model control group. Immunohistoehemistry measurements showed that the transforming growth factor beta1 protein expressions of rat asthmatic model group increased significantly by comparing with blank control group. Compared with asthmatic model control group, all the quantity of transforming growth factor beta1 decreased obviously in Liuwei Dihuang Granule group and positive control dexamethasone group. But there was no statistical difference between Liuwei Di- huang Granule group and blank control group in transforming growth factor beta1 expression. The ELISA de- tection showed that the change of transforming growth factor beta1 content in bronchoalveolar lavage fluid of every group was the same with the expressions of transforming growth factor betal detected by immunohisto- chemistry. Conclusion Traditional Chinese medicine Liuwei Dihuang Granule intervention can reduce the transforming growth factor beta1 expression in lung tissues of rat asthmatic models.

关 键 词：支气管哮喘 六味地黄颗粒 TGF-Β1 

分 类 号：R256.5[医药卫生—中医内科学]