2型糖尿病合并肾性高血压模型的建立及评价  

Establishment and Assessment of Model of Type 2 Diabetes Mellitus(T2DM)Complicated Renal Hypertension

作　　者：万斌[1] 孙丽薇[2] 张巧香[1] 张世玉[3] 罗烘权 顾为望[1] 

机构地区：[1]南方医科大学实验动物中心,广东广州510515 [2]中山大学中山医学院医学遗传学教研室,广东广州510080 [3]南方医科大学南方医院超声科,广东广州510515

出　　处：《动物医学进展》2016年第5期53-59,共7页Progress In Veterinary Medicine

基　　金：广东省科技基础条件建设项目(2013B060300001)

摘　　要：制备一种发病过程类似于人类2型糖尿病合并肾性高血压疾病的大鼠模型。大鼠高脂高糖膳食4周后,予快速腹腔注射链脲佐菌素(STZ)溶液28mg/kg,制作成2型糖尿病模型。2周后,连续3d均测得空腹血糖(FBG)≥7.8mmoL/L或随机血糖(RBG)≥16.7mmol/L,为2型糖尿病成模标准。待血糖稳定后,再用改良的"两肾一夹法"结扎肾动脉,造成一侧肾动脉狭窄,形成肾性高血压。2周后,连续3d均测得大鼠收缩压(SBP)≥140mmHg,同时FBG≥7.8mmol/L或RBG≥16.7mmol/L,确定为2型糖尿病合并肾性高血压模型制作成功。4周后,模型大鼠血糖、血压稳定。与空白对照组相比,复合模型组大鼠出现体重减轻、空腹血糖升高、糖化血红蛋白值升高(P<0.01),血压升高(P<0.01);与糖尿病组和假手术组相比,复合模型组大鼠血肌酐,尿素氮变化不明显(P>0.05),但血压明显升高(P<0.01);同时,彩超结果显示,复合模型组大鼠左侧肾脏、肾动脉直径均变小,血流速增快。大鼠高脂高糖膳食后,用低剂量链脲佐菌素(STZ)溶液腹腔注射,再用改良的"二肾一夹"法制备的2型糖尿病合并肾性高血压大鼠模型,在一定程度上较好地模拟出该疾病的发病特点,可为2型糖尿病合并肾性高血压后的临床及试验研究提供一个稳定、实用、有效的新模型。The rat model was induced to simulate the pathogenic process of type 2 diabetes mellitus（T2DM） complicated renal hypertension of human. The type 2 diabetic model of rats was prepared by the quick intraperitoneal injection of STZ at 28 mg/kg after high calorie and high sugar diet for 4 weeks. The type 2 diabetic model was established by the measurement FBG≥7.8 mmol/L or RBG≥16.7 mmol/L for successive 3 days after 2 weeks. After the blood sugar reached a steady value,the modeled of renal hypertention was prepared by using the improved method of ＂tow kidney one clip＂ to produce the renal artery stenosis of one side. The model of type 2 diabetes mellitus（T2DM） complicated renal hypertension was successfully replicated by the 3 daysr measurement, which showed that the rats＇ blood pressure SBP≥140 mmhg and FBG≥7.8 mmol/L or RBG≥16.7 mmol/L two weeks latter. After 4 weeks, the blood sugar and blood pressure of the model rats reached a steady level. Compared with the blank control group,the body weight of the complex model group were decreased, and the fasting glucose, GHbAlc and blood pressure significantly rosed （P〈0.01）. Compared with the diabetes group and the sham group,serum creatinine and urea nitrogen of the complex group showed no significant changes（P〉0.05） ,but a significant increase occurred in blood pressure occurred（P〈0.01）. The result of color ultrasonography showed a shrink of the left kid- ney and renal artery and a faster blood flow velocity. The model of type 2 diabetes mellitus （T2DM） complicated renal hypertension established by injecting low-dose STZ intraperitoneally after high fat and high glucose feed and by applying the improved method of ＂tow kidney one clip＂ can simulate the pathogenic characteristics of disease and provide a stable, practical and effective model for the clinic and experiment researches of type 2 diabetes mellitus（T2DM） complicated renal hypertension.

关 键 词：高脂高糖 链脲佐菌素(STZ) 2型糖尿病 肾性高血压 大鼠 

分 类 号：S852.3[农业科学—基础兽医学]