维生素C对红细胞氧化损伤保护作用研究  

Protective Effect of Vitamin C on Oxidative Damage of Erythrocytes

作　　者：李嘉欣[1] 钱多[2] 解彩霞[1] 苏燕[1] 

机构地区：[1]包头医学院生物化学与分子生物学教研室,内蒙古包头014060 [2]包头市第四医院检验科,内蒙古包头014030

出　　处：《动物医学进展》2016年第5期60-62,共3页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(81160214);内蒙古自然科学基金项目(2010BS1101);内蒙古自治区高等学校科学研究项目(NJZY14265);秦文斌科技教育基金项目(201309);内蒙古卫生厅科技计划项目(2010056)

摘　　要：为了利用血红蛋白释放试验(HRT)检测生理浓度维生素C(VC)对生理浓度过氧化氢(H2O2)氧化损伤后红细胞的保护作用,用终浓度为0、0.15、0.3、0.4、0.6、0.7、0.85mmol/L的VC溶液处理红细胞悬液并进行HRT。再分别用0、1.25、2.50、3.75、5.00mL/L的H2O2溶液处理0.4mmol/L VC作用后红细胞悬液(VC保护组)和VC未作用的红细胞悬液(对照组),测定红细胞相对溶解度。结果表明,VC达到生理浓度时,血红蛋白再释放量逐渐减弱;VC保护组红细胞相对溶解度与对照组差别不大。说明生理浓度的VC能减弱HRT的血红蛋白再释放,降低红细胞破膜率,但不能显著降低H2O2对细胞膜的氧化损伤。In order to use hemoglobin releasing test （HRT） to detect the protective effect of physiological concentration of vitamin C on erythrocytes treated by physiological concentration of hydrogen peroxide （H2O2）, The final concentrations of 0, 0.15, 0.3, 0.4, 0.6, 0. 7, 0.85 mmol/L vitamin C solution, were used to treat erythrocyte suspensions and HRT was conducted. Then 0, 1. 25,2. 50,3. 75,5. 00 mL/L H2O2 solutions were used to treat 0.4mmol/L vitamin C treated erythrocyte suspension （vitamin C protec- tion groups） and vitamin C untreated erythrocyte suspension （control groups） respectively,and the relative solubility of erythrocytes was measured. The results showed that the release of hemoglobin decreased gradually when the concentration of vitamin C reached to the physiological level. The relative solubility of erythrocytes in vitamin C protection groups had no significant difference from that of the control groups. It showed that the physiological concentration of vitamin C can decrease the release of hemoglobin by HRT, and decrease the rate of erythrocyte membrane breaking rate,but it can not significantly reduce the oxidative damage of H2O2 on the cell membrane.

关 键 词：维生素C 血红蛋白释放试验 过氧化氢 氧化损伤 

分 类 号：S852.21[农业科学—基础兽医学]