高效液相色谱法测定咳停合剂中吗啡含量  

Content Determination of Morphine in Anticough Mixture by HPLC

作　　者：冯静 余孟君 欧阳冰[1] 卢来春[1] 

机构地区：[1]中国人民解放军第三军医大学大坪医院野战外科研究所,重庆400042

出　　处：《中国药业》2016年第8期64-66,共3页China Pharmaceuticals

基　　金：中国人民解放军军队医疗机构制剂标准提高科研专项课题;项目编号:13ZJZ01-6

摘　　要：目的建立测定咳停合剂中吗啡含量的高效液相色谱（HPLC）法。方法色谱柱为Xtimate C8柱（Welch Xtimate,250 mm×4.6 mm,5μm）,流动相为乙腈-0.025 mol/L磷酸二氢钾-0.002 5 mol/L庚烷磺酸钠（30∶85∶85,V/V/V）,检测波长为220 nm,流速为1.0 m L/min,进样量为20μL。结果吗啡质量浓度在1.008~20.16μg/m L范围内与峰面积呈良好的线性关系,r=0.999 7（n=7）,平均回收率为99.16%,RSD=1.28%（n=9）。结论该方法检测结果准确可靠,灵敏度高,可作为咳停合剂中吗啡的含量测定方法。Objective To establish an HPLC method of determining the content of morphine in Anticough Mixture. Methods The HPLC was used with a Welch Xtimate C8 column（250 mm ×4. 6mm,5 um）,the mobile phase consisted of aeetonitri1-0. 025 mol/L potassi- um dihydrogen phosphate solution-0. 002 5 mol/L sodium heptane- 1-sulphonate （30：85：85, V/ V/ V） with a flow rate of 1.0 mL/min,the detective wavelength was 220 nm and the injection volume was 20uL. Results The morphine showed good linear rela- tionship with the peak area in the range of 1.008-20. 16ug/mL, r=0.999 7（n=7）,and the average recovery rate was 99.16%, RSD = 1.28% （n =9）. Conclusion The method is accurate and high sensitivity,it is suitable for the determination of morphine in Antieough Mixture.

关 键 词：高效液相色谱法 咳停合剂 吗啡 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]