γδT细胞TCR分子与Mtb-Ag表位结合的初步探讨  

Primary Discussion for the Specific Binding of TCR Molecule on Human γδT Cells with Mycobacterium Tuberculosis Peptide Antigen

作　　者：张晖[1] 薛洪宝[1] 

机构地区：[1]蚌埠医学院化学教研室,安徽蚌埠233030

出　　处：《河西学院学报》2016年第2期97-101,共5页Journal of Hexi University

基　　金：安徽省高校自然科学研究重点项目(KJ2016A485);安徽省高校自然科学研究一般项目(KJ2015B037by);安徽省高校省级优秀青年人才基金重点项目(2013SQRL051ZD);安徽省高等教育振兴计划重大教学改革研究项目(2015zdjy101)

摘　　要：探讨采用毛细管电泳(CE)法分离检测结核杆菌抗原(Mtb-Ag)的活性成分能与γδT细胞TCR分子进行表位结合.试验温度25℃;电泳毛细管:熔融石英毛细管55cm(40cm处检测窗口)×50μm i.d.;进样压力0.5 psi;进样时间5.0s;缓冲溶液浓度为0.05mol·L^(-1)(p H=10.20)的乙酸钠溶液;分离电压+15 k V.该方法能依据相对分子质量大小分离Mtb-Ag样品活性成分.样品加标回收率为96.75%,相对标准偏差低于7.45%.采用CE法分离检测Mtb-Ag样品中的活性成分能与γδT细胞TCR分子进行表位结合.The research objective is to preliminarily discuss the specific binding of TCR molecule on human γδT cells with active ingredients separated from Mycobacterium tuberculosis peptide antigen by CE. The analyses are carried out on an P/ACETM MDQ capillary electrophoresis system （Beckman Coulter, USA） equipped with an UV-Vis detector. Separation was performed in a bare fused-silica capillary which was 55.0 cm long （40.0 cm to the detector, 50μm i.d.） at 25℃, and a 0.05mol·L^-1 sodium acetate solution （pH=10.20 is used as background electrolyte. The samples are introduced into the capillary under a pressure of 0.5 psi for 5.0s, and the separation voltage is set at ＋15 kV. The detection wavelength is fixed at 200 nm. Several active ingredients are separated from Mtb-Ag basically according to the molecular weight. The spiked recoveries are about 96.75%, and the relative standard deviation is kept within 7.45%. The specific binding of TCR molecule on human ℃ γδT cells with active ingredients separated occurred. from Mycobacterium tuberculosis peptide antigen by CE

关 键 词：ΓΔT细胞 结核杆菌抗原(Mtb-Ag) 表位结合 毛细管电泳法 

分 类 号：Q5-33[生物学—生物化学]