丹参酮ⅡA抑制过度自噬减轻心肌细胞缺氧/复氧损伤（英文）  

Effects of Tanshinone ⅡA on cardiomyocytes against hypoxia/reoxygenation injury by inhibiting excessive autophagy

作　　者：于佳慧[1] 王玲燕[1] 李妍妍[1] 朱彦[1] 樊官伟[1] 

机构地区：[1]天津中医药大学中医药研究院,天津300193

出　　处：《中华中医药杂志》2016年第5期1689-1694,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：National Key Basic Research Program of China(973 Program)(No.2012CB518404)~~

摘　　要：目的:探究丹参主要成分丹参酮ⅡA能否减轻心肌细胞缺氧/复氧(H/R)损伤,以及对其诱导的自噬相关机制的研究。方法:在乳鼠心肌细胞原代培养的基础上,构建缺氧6h复氧18h的细胞损伤模型。应用丹参酮ⅡA预处理,检测细胞活力、m RFP-GFP-LC3双荧光标记的自噬潮的变化及自噬相关蛋白P62、LC3Ⅱ、Beclin-1及Bcl-2的表达。结果:H/R处理引起细胞损伤甚至死亡。给予丹参酮ⅡA预处理后,可以显著改善心肌细胞活性,抑制自噬潮,并降低由H/R引起的自噬相关蛋白P62、LC3Ⅱ及Beclin-1的表达,同时增强抗凋亡蛋白Bcl-2的表达。在加入100nmol/L的Rapamycin处理后,上述蛋白的表达受到抑制。结论:丹参酮ⅡA可能是通过激活m TOR信号通路抑制过度自噬来减轻心肌细胞缺氧/复氧损伤的。Objective： To investigate the effect of Tanshinone ⅡA（major component of Salvia miltiorrhiza Bge.） on cardiomyocytes against hypoxia/reoxygenation（H/R） injury and potential molecular mechanisms involved autophagy in cardiomyocytes. Methods： Neonatal rat cardiomyocytes were cultured and subjected to 6h-hypoxia followed by 18h-reoxygenation to induce cell damage. Cardiomyocytes were pretreated with different concentration of Tanshinone ⅡA before cell viability was assessed with the CCK8 assays. Autophagy flux in mR FP-GFP-LC3-transfected cells was evaluated with confocal and western blotting was applied to assess the autophagic and apoptotic components expression including P62, LC3 Ⅱ, Beclin-1 and Bcl-2. Results： H/R remarkably damaged cardiomyocytes or even caused cell death. However, pretreatment of Tanshinone ⅡA significantly improved cell survival and protected against H/R induced cell injury, inhibited autophagy fluxand decreased the expression of autophagy related proteins like P62, LC3 Ⅱ, Beclin-1 and increased the expression of antiapoptosis protein Bcl-2, these aforementioned proteins were found to be down-regulated following 100nmol/L Rapamycin treatment. Conclusion： Tanshinone ⅡA protects cardiomyocytes against H/R injury by inhibiting excessive autophagy through m TOR activation.

关 键 词：丹参酮ⅡA 缺氧/复氧损伤 自噬 急性心肌梗死 

分 类 号：R285.5[医药卫生—中药学]