龟板改善激素性骨质疏松大鼠骨量、骨微细结构、骨生物力学和骨代谢的机制探讨  

Mechanism of Carapax et Plastrum Testudinis improving bone mass, microarchitecture, biomechanics and bone metabolism

作　　者：任辉[1] 张志达[1] 梁德[2] 沈耿杨[1] 丘婷[1] 林顺鑫 姚珍松[2] 江晓兵[2] 庄洪[2] 

机构地区：[1]广州中医药大学,广州510405 [2]广州中医药大学第一附属医院脊柱骨科,广州510405

出　　处：《中华中医药杂志》2016年第5期1858-1862,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81503591);广东省科技项目(No.2014A020221021);广东省自然科学基金(No.2014A030310082);广东省教育厅学科建设专项基金(育苗工程)(No.2013LYM-0012);广州中医药大学优秀青年学者科研基金项目(No.KAB110133K04);广州中医药大学及第一临床医学院优秀博士论文培育项目(No.YB201501)~~

摘　　要：目的:研究补肾中药龟板对激素性骨质疏松大鼠骨量、骨微细结构、骨生物力学和骨代谢指标的改善作用,并对其分子机制进行探讨。方法:40只4月龄SD大鼠随机分为4组:空白组、模型组、阿伦磷酸钠组和龟板组。模型组、阿伦磷酸钠组及龟板组皮下注射地塞米松造模,成功后分别用0.9%氯化钠溶液、阿伦磷酸钠和龟板进行灌胃。12周后取大鼠腰椎(L1-6)和血清进行检测:双能X线检测腰椎骨量、micro-CT检测腰椎骨微细结构、压缩实验检测腰椎生物力学,HE染色观察腰椎形态学改变,ELISA检测大鼠PINP和β-CTX水平表达,q PCR检测腰椎Runx2、SP-7、CTSK和AP-1的m RNA表达。结果:空白组、龟板组及阿伦磷酸钠组骨密度、骨矿物质含量、骨表面积、骨小梁数量、骨小梁厚度、压缩强度明显高于模型组(P<0.05,P<0.01),骨小梁间距、PINP和β-CTX水平、CTSK m RNA表达水平明显低于模型组(P<0.05,P<0.01)。结论:补肾中药龟板可能通过降低骨转换有效改善激素性骨质疏松;CTSK可能是龟板改善激素性骨质疏松大鼠骨量、骨微细结构、骨生物力学和骨代谢的作用靶点之一。Objective： To study the improving effect of kidney-invigorating Chinese herbal Carapax et Plastrum Testudinis on bome mass, microarchitecture, biomechanics and bone metabolism, and explore the associated molecular mechanism. Methods： Forty 4-month SD rats were randomly divided into four groups： control group, model group, alendronate group and Carapax et Plastrum Testudinis group. Model group, alendronate group and Carapax et Plastrum Testudinis group received injection of dexamethasone, and then treated with normal saline, alendronate and Carapax et Plastrum Testudinis by gavage for each group. Lumbar and serum were collected for analysis after 3 months. Bone mass of lumbar was detected by DEXA, microarchitecture of lumbar was detected by micro-CT, biomechanics of lumbar was detected by compressive test, morphological change of lumbar was observed by HE method, the expression of PINP and β-CTX were detected by ELISA and m RNA expression of Runx2, SP-7, CTSK and AP-1 were detected by PCR. Results： The BMD, BMC, AREA, Tb.N, Tb.Th, and compressive strength in control, alendronate and Carapax et Plastrum Testudinis groups were higher than model group（P〈0.05, P〈0.01）, while Tb.Sp, PINP and β-CTX were lower than model group（P〈0.05, P〈0.01）. Conclusion： Carapax et Plastrum Testudinis might reverse GIOP by reducing bone turnover markers; and CTSK might be one of the targets of Carapax et Plastrum Testudinis improving bone mass, microarchitecture, bone strength, histological structure and bone turnover in molecular level.

关 键 词：激素性骨质疏松 龟板 MICRO-CT 骨生物力学 骨微细结构 骨代谢 分子机制 

分 类 号：R285.5[医药卫生—中药学]