芍药内酯苷对环磷酰胺致血虚小鼠的补血作用及机制  

Effects and mechanism of albiflorin on mouse model of blood deficiency syndrome induced by cyclophosphamide

作　　者：朱映黎[1] 王林元[1] 王景霞[1] 王成龙[1] 赵丹萍[1] 王子晨[1] 张建军[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中华中医药杂志》2016年第5期1892-1896,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81473370;No.81173569)~~

摘　　要：目的:研究芍药内酯苷对环磷酰胺致血虚小鼠的补血作用及机制。方法:以四物颗粒及芍药苷为对照,检测外周血象,胸腺与脾脏指数,骨髓病理切片,酶免法检测血浆粒细胞集落刺激因子(G-CSF)、放免法检测血清粒细胞-巨噬细胞集落刺激因子(CM-CSF)、肿瘤坏死因子-α(TNF-α)含量变化;RT-q PCR法检测脾脏中G-CSF、CM-CSF及TNF-α的基因表达;Western Blot法检测骨髓中G-CSF、CM-CSF及TNF-α的蛋白表达。结果:结果:与模型组比较,芍药苷组与芍药内酯苷高剂量组均能明显升高白细胞数和胸腺指数(P<0.01,P<0.05),升高G-CSF与GM-CSF含量(P<0.01,P<0.05),降低血清中TNF-α水平(P<0.05,P<0.01),降低脾脏TNF-αm RNA的表达(P<0.05)。病理切片结果显示,芍药苷组与芍药内酯苷高剂量组小鼠骨髓细胞数量多,密度大,巨核细胞数量与模型组相比较少。结论:芍药内酯苷具有补血作用,不仅能够通过促进造血生长因子的分泌调节造血细胞的增殖分化,还能够调控负向造血因子,减轻其对造血功能的抑制。Objective： To study the effect and mechanism of albiflorin on mouse model of blood deficiency syndrome induced by cyclophosphamide. Methods： Comparing with Siwu granules and albiflorin, the peripheral hemogram, the index of thymus gland and spleen were detected, the levels of G-CSF, TNF-α and GM-CSF in plasma or in serum were measured by pathological section of bone marrow and RIA. The m RNA expressions of hematopoietic-related factors in spleen were detected by RT-q PCR. In addition, the protein levels of haemopoietic factors in bone marrow were detected by Western Blot. Results： Compared with the model group, the level of WBC, the index of thymus gland, the levels of GM-CSF and G-CSF in albiflorin group were increased（P〈0.05, P〈0.01）, the marrow pathology was improved, the content of TNF-α in serum and the expression of TNF-α m RNA in spleen were decreased（P〈0.01, P〈0.05）. The protein expressions of G-CSF, GM-CSF had the trend of rising, but no statistical difference. Conclusion： Albiflorin had the activity of replenishing blood. It could not only regulate the proliferation and differentiation of hematopoietic cells by promoting the secretion of hematopoietic growth factors, but also control the negative hematopoietic factor to reduce the inhibition of hematopoiesis.

关 键 词：芍药内酯苷 芍药苷 环磷酰胺法 血虚证 造血因子 

分 类 号：R285.5[医药卫生—中药学]