表皮生长因子对化疗损伤小鼠骨髓基质细胞的保护作用  

Protective effect of epidermal growth factor on the chemotherapy-injured mouse bone marrow stromal cells

作　　者：刁远明[1,2] 詹瑧[2] 周韬[2] 张伟伟[2] 张李唯 董伟[2] 张军峰[2] 

机构地区：[1]广州中医药大学基础医学院,广东广州510006 [2]南京中医药大学基础医学院,江苏南京210023

出　　处：《中国医药导报》2016年第12期8-11,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81473593;81473458);广东省高等学校优秀青年教师培养计划项目(YQ2015042)

摘　　要：目的探讨表皮生长因子(EGF)对5-氟尿嘧啶(5-FU)化疗损伤小鼠骨髓基质细胞(OP9)的保护作用。方法首先建立5-FU化疗损伤OP9细胞模型并摸索EGF作用剂量。然后,将实验分为空白对照组、模型组、实验组。用25μg/m L 5-FU作用OP9细胞作为模型组,用20 ng/m L的EGF处理25μg/m L 5-FU损伤的OP9细胞作为实验组,并设常规培养的OP9细胞作为空白对照组。分别用CCK-8法检测细胞存活率,流式细胞术(FCM)检测细胞凋亡,一氧化氮(NO)测试盒、一氧化氮合酶(NOS)测定试剂盒、Caspase-3活性检测试剂盒分别检测NO、i NOS、TNOS及Caspase-3的含量。结果与空白对照组比较,模型组细胞存活率明显降低(P<0.01);与模型组比较,EGF可以显著保护5-FU损伤的OP9细胞,使其存活率升高(P<0.01)。与空白对照组比较,模型组细胞早期凋亡率明显提高(P<0.05),Caspase-3含量明显增加(P<0.05),NO生成明显增多(P<0.05),i NOS表达明显上升(P<0.05);与模型组比较,实验组化疗损伤OP9细胞的早期凋亡率显著降低(P<0.05),Caspase-3含量明显下降(P<0.05),i NOS的表达明显受到抑制(P<0.01),NO的生成明显减少(P<0.05)。结论 EGF可能通过降低Caspase-3含量抑制细胞凋亡,减少NO的过度生成,发挥其细胞保护作用。Objective To investigate the protective effect of epidermal growth factor （EGF） on 5-Fluorouracil （5-FU） chemotherapy-injured mouse bone marrow stromal cells （OP9）. Methods First, the OP9 injury model was established by using 5-FU and the effect of EGF on OP9 injury model was explored. Then, the experiment was divided into blank control group, model group and experimental group. The OP9 cells treated with 25 Ixg/mL 5-FU were used as the mod- el group. The model cells treated with 20 ng/mL EGF were used as the experimental group, and the normal OP9 cells were used as blank control group. CCK-8 assay was used to detect cell viability. Cell apoptosis was detected by FCM. NO, iNOS, TNOS, Caspase-3 were detected by nitric oxide （NO） test kit, nitric oxide synthase （NOS） assay kit, Cas- pase-3 active assay kit. Results Compared with the blank control group, the cell survival rate of model group was sig- nificantly decreased （P 〈 0.01）, and compared with the model group, EGF could significantly protect OP9 cells from 5-FU damage, and increased the survival rate of cells （P 〈 0.01）. Compared with the blank control group, the early apoptosis rate of model group was significantly enhanced （P 〈 0.05）, the content of Caspase-3 was significantly increased （P 〈 0.05）, the NO production was significantly increased （P 〈 0.05）, and the expression of iNOS was significantly rose （P 〈 0.05）. Compared with the model group, the early apoptosis rate of injury OP9 cells of the experimental group was significantly decreased （P 〈 0.05）, the content of Caspase-3 was significantly reduced （P 〈 0.05）, the iNOS expression was significantly inhibited （P 〈 0.01）, and NO generation was lessened （P 〈 0.05）. Conclusion EGF might inhib- it apoptosis through decreasing the content of Caspase- 3 and reducing the excessive production of NO to play its role in cell protection.

关 键 词：表皮生长因子 5-氟尿嘧啶 OP9细胞 化疗损伤 

分 类 号：R392.12[医药卫生—免疫学]