基于ISSR标记的新疆伊犁薰衣草品种资源的遗传多样性分析  

Genetic Diversity of Distinctive-spicy Lavender Cultivars in Xinjiang Yili Revealed by ISSR Analysis

作　　者：郭丹丽[1] 李敏[1] 王自健[1] 蒋新明[1] 黄先忠[2] 路喆[1] 王朴[1] 

机构地区：[1]新疆生产建设兵团第四师农业科学研究所,新疆伊宁835000 [2]石河子大学生命科学学院植物基因组学实验室,新疆石河子832003

出　　处：《新疆农业科学》2016年第4期716-720,共5页Xinjiang Agricultural Sciences

基　　金：兵团青年基金项目(2014CD007);兵团科技支疆项目(2014AB010)~~

摘　　要：【目的】揭示不同薰衣草品种的遗传多样性。【方法】利用ISSR分子标记技术对45个薰衣草品种的亲缘关系进行分析。【结果】45份材料DNA共扩增出83条谱带,平均每条引物扩增出9.2条带,多态性为60条带,多态性比例为72.29%。45份薰衣草品种两两之间的遗传距离值在0.663~0.988。群体的有效等位基因数（Ne）、基因多样度（H）、Shannon信息指数（I）分别为1.399 5、0.240 4和0.364 3。45个薰衣草品种在遗传距离0.72处可分为两类,第Ⅰ类包含44个品种,第Ⅱ类仅有1个品种。【结论】伊犁地区的45个薰衣草品种遗传多样性水平低,亲缘关系较近。[ Objective] This research focuses on assessment of the genetic variability and diversity of lav- ender （Lavandula pedunculata）. [ Method] Genetic relations among 45 cuhivars of lavenders were analyzed by inter- simple sequence repeat （ISSR） makers. [ Result] A total of 83 ISSR bands were obtained, among which 60 were polymorphic bands. On average, amplification band of the each primer was 9.2 and polymor- phism ratio was 72.29%. The dissimilarity coefficient of the 45 lavender cultivars ranged from 0. 663 to 0. 988. The efficient number of alleles, Nei~ genetic diversity, and Shannon~ information index of the culti- vars varieties were 1. 399,5, 0. 240,4 and 0.364,3, respectively. The 45 lavender cultivars were divided into 2 groups at the genetic distance 0.72 by UPGMA. The first group contained 44 cultivars and the second group only contained 1 cultivars. [ Conclusion] The population genetic diversity of 45 lavender cultivars was high and the genetic relationship was close.

关 键 词：伊犁薰衣草 ISSR标记 聚类分析 遗传多样性分析 

分 类 号：S635.1[农业科学—蔬菜学]