糖尿病大鼠骨骼肌内脂素和磷脂酰肌醇3激酶的表达  

The expression of visfatin and phosphatidylinositol 3-kinase isoforms in skeletal muscle of diabetic rats

作　　者：张晗[1] 张安星[1] 高琳[1] 阳琰[1] 廖鑫[1] 李明泽[1] 杨孟雪[1] 

机构地区：[1]遵义医学院附属医院内分泌科,贵州遵义563003

出　　处：《重庆医学》2016年第14期1889-1892,共4页Chongqing medicine

基　　金：贵州省社会发展攻关项目[SY(2013)3033];贵州省科技合作项目[(2010)7004]

摘　　要：目的观察糖尿病大鼠骨骼肌中内脂素(visfatin)、磷脂酰肌醇3激酶(PI3K)的表达,探讨visfatin在糖尿病中的作用及可能机制。方法 8周龄雄性SD大鼠55只(造模成功39只),采用随机数字表法分为4组:正常对照组(A组)10只,饮食诱导肥胖组(B组)10只,血糖未控制糖尿病组(C组)10只,血糖控制糖尿病组(D组)9只。喂养至12周末,测定空腹血糖(FBG)、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(FFA)、空腹胰岛素(FINS),计算稳态模型评估的胰岛素抵抗指数(HOMA-IR)及胰岛素敏感指数(ISI);RT-PCR测定骨骼肌组织visfatin、PI3K mRNA表达;Western blot测定骨骼肌组织visfatin、PI3K蛋白表达。结果 C组FBG较A、B组明显增高(P<0.01);D组FBG较C组明显降低(P<0.01)。B组TG较A组明显增高(P<0.05);C组TG、TC、LDL-C、FFA较A、B组明显增高(P<0.01);D组TG、TC、FFA较A组明显增高(P<0.05);D组TG较A、B组明显增高(P<0.05);D组TG、TC、LDL-C、FFA较C组明显降低(P<0.01)。B、C组较A组FINS、HOMA-IR明显增高,ISI明显降低(P<0.01),C组较B组FINS、HOMA-IR明显增高,ISI明显降低(P<0.01)。C、D组visfatin mRNA和蛋白表达较A组均明显增高(P<0.05),C组visfatin蛋白较B组明显增高(P<0.01)。C组PI3K mRNA和蛋白表达较A、B组均明显降低(P<0.01),D组PI3K mRNA和蛋白表达较A组均明显降低(P<0.05)。结论 visfatin在骨骼肌中的表达水平与骨骼肌糖脂代谢状态有关,可能通过PI3K参与骨骼肌糖脂代谢的调节。Objective To observe the expression of visfatin and phosphatidylinositol 3-kinase isoforms（PI3K）in skeletal muscle of diabetic rats,and to explore the function and the possible mechanism of visfatin in diabetes mellitus.Methods Thirtynine 8-week-old male SD rats were randomly divided into four groups which were normal control group（group A,n=10）,diet-induced obesity group（group B,n=10）,blood glucose not control diabetes group（group C,n=10）and blood glucose control diabetes group（group D,n=9）.After 12 weeks fed,FBG,triglyceride（TG）,total cholesterol（TC）,low density lipoprotein cholesterol（LDL-C）,free fat acid（FFA）and fasting insulin（FINS）were measured.The homeostasis model assessment（HOMA-IR）and insulin sensitivity index（ISI）were calculated.The expression of visfatin and PI3 K mRNA in skeletal muscle tissue were detected by RT-PCR,and the expression of visfatin and PI3 Kprotein were determined by Western blot.Results FBG of group C was significantly higher than those of group A and B（P〈0.01）;FBG of group D was significantly lower than that of group C（P〈0.01）;TG of group B was significantly higher than that of group A（P〈0.05）.TG,TC,LDL-C,FFA of group C was significantly higher than those of group A and B（P〈0.01）;TG,TC,FFA of group D was significantly higher than those of group A（P〈0.05）;TG of group D was significantly higher than those of group A and B（P〈0.05）;TG,TC,LDL-C,FFA of group D was significantly lower than those of group C（P〈0.01）.Comparing with group A,FINS,HOMA-IR of group B and C were significantly higher and ISI were significantly lower（P〈0.01）;comparing with group B,FINS,HOMA-IR of group C was significantly higher and ISI was significantly lower（P〈0.01）.The expression of visfatin mRNA and protein in skeletal muscle of group C and D were significantly higher than those of group A（P〈0.05）,while visfatin protein of group C was significantly higher than that of group B（P〈0.01）.The expression o

关 键 词：1-磷脂酰肌醇3-激酶 糖尿病 肥胖症 肌 骨骼 内脏脂肪素 SD大鼠 

分 类 号：R587.1[医药卫生—内分泌]