趋化因子受体D6、DARC在人乳腺癌组织中的表达研究  

Study of D6 and DARC expression in breast cancer

作　　者：景周宏[1] 曹勇[1] 熊忠讯[1] 江歌丽[1] 刘晓渝[1] 曾晓华[1] 

机构地区：[1]重庆市肿瘤研究所乳腺外科,400030

出　　处：《重庆医学》2016年第14期1915-1917,共3页Chongqing medicine

基　　金：重庆市卫生局重点项目(2012-1-125)

摘　　要：目的研究乳腺癌组织中趋化因子受体D6与Duffy抗原趋化因子受体(DARC)表达水平,探讨与乳腺癌临床分期及相关病理指标之间的关系。方法收集乳腺癌患者癌组织标本120例,均为病理确诊,应用免疫组织化学SP法检测在各标本中D6和DARC表达水平,统计分析D6、DARC蛋白表达与乳腺癌临床分期、淋巴结转移、雌激素受体(ER)、孕激素受体(PR)、人表皮生长因子受体-2(HER-2)表达的相关性。结果 D6、DARC在乳腺癌及癌旁正常乳腺中均有表达,癌旁正常乳腺组织中阳性表达率分别为71.7%、68.3%,在乳腺癌的阳性表达率分别为68.3%、65.0%。D6、DARC的表达情况与乳腺癌临床分期及是否发生腋淋巴结转移密切相关(P<0.01)。D6、DARC的表达与患者ER、PR及HER-2的表达等病理指标无关(P>0.05)。结论D6、DARC在乳腺癌发展中呈现负性调节作用,其在乳腺癌中表达的高低与HER-2、ER及PR等临床病理指标无明显相关性。Objective To observe and study the expression of chemokine D6 and DARC in breast cancer,and to analyse their association with cTNM stage and pathological features of breast cancer.Methods Expressions of chemokine D6 and DARC in 120 breast cancer patients were determined by IHC（immunohistochemistry）technique（SP）.The corresponding normal breast tissues in paraneoplastic were also detected.The level of D6 and DARC in 120 breast cancer were analysed whether it was associated with cTNM stage,axillary lymph node status,ER status,PR status and HER-2status.Results D6 and DARC were expressed in both breast cancer and normal breast tissues in paraneoplastic.The positive expression rate of D6 and DARC in normal paraneoplastic breast tissues were 71.7% and 68.3%.The positive expression rate of D6 and DARC in breast cancer were 68.3% and 65.0%.The expression of DARC and D6 was closely related to the clinical stage of breast cancer and whether the axillary lymph node metastasis occurred（P〈0.01）.The expression of D6 and DARC were not associated with ER,PR and HER-2status（P〈0.05）.Conclusion The level of D6 and DARC expression might play negative regulatory role in breast cancer staging.The level of expression is not significantly correlated with pathological features of breast cancer.

关 键 词：乳腺肿瘤 免疫组织化学 淋巴转移 受体 趋化因子 DUFFY抗原趋化因子受体 D6 表达 

分 类 号：R737.9[医药卫生—肿瘤]