屋尘螨变应原对人肥大细胞脱颗粒的影响  

Effect of house duct mite on the degranulation of the mast cells

作　　者：唐慧[1] 王朵勤[1] 沈燕芸[1] 徐金华[1] 

机构地区：[1]复旦大学附属华山医院皮肤科,上海200040

出　　处：《上海医药》2016年第9期10-13,共4页Shanghai Medical & Pharmaceutical Journal

摘　　要：目的:阐明屋尘螨变应原Derp1对人肥大细胞HMC-1释放类胰蛋白酶水平的影响。方法:流式细胞法测定HMC-1细胞表面蛋白酶激活受体2(PAR2)表达水平;将Derp1、SLIGRL-NH_2(PAR2激活剂)、LRGILS-NH_2(PAR2对照肽)及Derp1+FSLLY(PAR2拮抗剂)分别处理HMC-1细胞,用ELISA检测药物刺激后类胰蛋白酶水平;采用钙绿实验检测PAR2生物学功能。结果:肥大细胞表面表达PAR2受体;Derp1单独作用于HMC-1可明显引起HMC-1细胞内钙流释放及类胰蛋白酶的释放,类胰蛋白酶释放水平明显高于SLIGRL-NH_2及正常对照;将Derp1与FSLLY共同作用于HMC-1时,可部分抑制HMC-1细胞内的钙流释放及类胰蛋白酶的释放。结论:屋尘螨变应原Derp1可以通过激活人肥大细胞HMC-1表面PAR2受体引起肥大细胞脱颗粒释放类胰蛋白酶。Objective： To study the effects of the house dust mite allergen Derpl on the secretion of tryptase from the human mast cell line HMC-1. Methods： Flow cytometry assay was performed to determine the expression levels of protease activated receptor 2 （PAR2） on the surface of HMC-1 cells. HMC-1 cells were treated with Derpl, SLIGRL-NH2 （PAR2 agonist）, LRGILS-NH2 （control peptide for PAR2） or Derp 1 ＋ FSLLY （PAR2 antagonist）, respectively, then the tryptase levels were measured using enzyme-linked immunosorbent assay （ELISA）. Biological functions of PAR2 were determined using the calcium green indicator. Results： PAR2 receptor was expressed on the surfaces of mast cells. Derpl alone induced a significant release of intracellular calcium and tryptase in HMC-1 cells. The level of tryptase release was significantly higher compared with the SLIGRL-NH2 treatment group and the normal control group. The combination of Derpl with FSLLY could partly inhibit intracellular calcium and tryptase release in HMC-1 cells. Conclusion： Derpl can induce HMC-1 cells degranulation to release tryptase by activating the PAR2 receptor on the cell surface.

关 键 词：屋尘螨 肥大细胞 蛋白酶激活受体2 类胰蛋白酶 

分 类 号：R593.1[医药卫生—内科学]