基于多指标权重分析和正交设计法优选白黄泄热止痢片复方提取工艺  

Exploration on extraction process of Compound Baihuang Xiere Zhili Tablets based on orthogonal design and multi-index weight analysis

作　　者：贾成友[1,2] 李微[1] 张传辉[2] 王云红[2] 蒋瑶[2] 陈娇[1] 赵凤平[1] 杨荣平[1,3] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]重庆市中药研究院,重庆400065 [3]西南大学药学院,重庆400715

出　　处：《中草药》2016年第6期917-922,共6页Chinese Traditional and Herbal Drugs

摘　　要：目的确定指标权重,优选白黄泄热止痢片复方提取工艺,为其工业化生产提供参考。方法以白头翁皂苷B4、盐酸小檗碱、秦皮甲素、秦皮乙素、东莨菪内酯溶出量和出膏率为评价指标,采用层次分析法（AHP）、CRITIC法及AHP-CRITIC混合加权法确定各指标权重系数,结合正交试验结果优化复方醇提工艺参数。结果白头翁皂苷B4、盐酸小檗碱、秦皮甲素、秦皮乙素和东莨菪内酯分别在121.1~1211.0、82.2~822.0、22.3~223.0、15.42~154.2、1.6~16.0μg/m L内呈良好的线性关系（r为0.999 7~0.999 9）,加样回收率为99.23%~100.63%,RSD均小于3.0%;AHP-CRITIC混合加权法较AHP法和CRITIC法科学、合理、稳定,按照其确定的权重系数进行综合评价确定的复方最佳提取工艺条件为处方药材加8倍量60%乙醇,提取3次,每次2.0 h。结论 AHP-CRITIC混合加权法确定的权重系数客观、真实,可以反映处方配伍信息;优选得到的工艺稳定可行,重复性好,可用于工业化生产。Objective To provide a reference for the industrial production of the extraction process of Compound Baihuang Xiere Zhili Tablets （CBXZT） by exploring the optional extraction process parameters based on determining the index weight. Methods With the extraction capacity of anemoside B4, berberine hydrochloride, aesculin, aesculetin, scopoletin, and ratio of extraction as comprehensive evaluation indexes, the weighting coefficient in six synthetic evaluation indexes was determined by Analytic Hierarchy Process （AHP）, Criteria importance through intercriteria correlation （CRITIC） method, and mixed weighted AHP-CRITIC. And ethanol-extracting process was optimized by orthogonat test. Results The excellent linearity with correlation coefficients were 121.1-1211.0, 82.2- 822.0, 22.3-223.0, 15.42-154.2, and 1.6-16.0 μg/mL, r of0.9990-0.999 9 was obtained, and the average recoveries of the five compounds were 99.23%-100.63% with all RSD values less than 3.0%. Mixed weighted AHP-CRITIC was more scientific, reasonable, and stable than AHP and CRITIC. The optimal process parameters were confirmed to be decocting for thrice with 2 h in each by eight times the amount of 60% ethanol, according to the weight coefficient determined by comprehensive evaluation. Conclusion These optimized technologies are stable, feasible, and suitable for standardized production of CBXZT. The weight coefficient determined by mixed weighted AHP-CRITIC was objective, real and could reflect the compound compatibility information. These optimized technologies are stable, feasible and suitable for standardized production of CBXZT.

关 键 词：白黄泄热止痢片 指标 权重分析 正交设计 提取工艺 层次分析法 CRITIC法 AHP-CRITIC混合加权法 

分 类 号：R284.2[医药卫生—中药学]