蝙蝠葛酚性碱对局灶性脑缺血大鼠海马神经细胞谷氨酸转运体EAAC1 mRNA表达的影响  

Effect of Phenolic Alkaloids from Menispermum dauricum on EAAC1 mRNA Expression in Hippocampal Neurons of Rats with Focal Cerebral Ischemia

作　　者：瞿小兰[1] 陈诚[2] 卢云[2] 罗攀[2] 郭莲军[2] 

机构地区：[1]武汉工程大学邮电与信息工程学院化工与制药教研室,武汉430073 [2]华中科技大学同济医学院药理学系,武汉430030

出　　处：《医药导报》2016年第5期444-447,共4页Herald of Medicine

摘　　要：目的探讨蝙蝠葛酚性碱(PAMd)对大鼠局灶性脑缺血后海马神经细胞谷氨酸转运体EAAC1 mRNA表达的影响,及其对脑缺血的保护机制。方法 SD大鼠42只,随机分为假手术组(不给药)、模型对照组(不给药)、PAMd组(灌胃给予PAMd 10 mg·kg-1),每组14只。线栓法制备大鼠局灶性脑缺血模型。采用2,3,5-氯化三苯基四氮唑(TTC)染色检测脑梗死体积,逆转录-聚合酶链反应(RT-PCR)检测海马神经细胞谷氨酸转运体EAAC1 mRNA表达。结果缺血24 h后,假手术组、模型对照组和PAMd组脑梗死比例分别为(0.0±0.0)%,(35.3±2.9)%,(21.3±3.8)%(P<0.05);缺血侧海马EAAC1 mRNA相对表达量分别为0.97±0.04,2.46±0.13,1.91±0.15(P<0.05)。结论 PAMd可能通过下调缺血后EAAC1的表达升高,从而减轻谷氨酸的兴奋毒性作用,发挥对脑缺血的保护作用。Objective To investigate the effect of phenolic alkaloids from Menispermum dauricum（ PAMd）on the mRNA expression of the glutamate transporter EAAC1 in hippocampal neurons of rats subjected to focal cerebral ischemia and to elucidate its neuroprotective mechanisms. Methods A total of 42 Sprague Dawley（SD）rats were randomly divided into three groups：sham group,model control group,and PAMd（10 mg＆#183;kg^-1 ,i.g）group（n = 14 each）.The focal cerebral ischemia model of rat was induced by the middle cerebral artery occlusion（ MCAO）. The 2,3,5-triphenyltetrazolium chloride（ TTC） staining was applied to measure the cerebral infarct size and the reverse transcriptase-polymerase chain reaction（RT-PCR）assay to detect EAAC1 mRNA expression in hippocampal neurons. Results After 24 h ischemia,the cerebral infarct volumes were （0.0±0.0）%,（35.3±2.9）% and（21.3±3.8）% in sham group,model control group and PAMd group,respectively（P〈0.05）. The relative expression levels of EAAC1 mRNA were 0.97±0.04,2.46±0.13,and 1.91±0.15 in the three groups,respectively （P〈0. 05）. Conclusion PAMd may protect against cerebral ischemia by up-regulating EAAC1 mRNA expression and alleviating the excitotoxicity of glutamic acid.

关 键 词：蝙蝠葛酚性碱 缺血 脑 谷氨酸转运体 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]