ZTC1+1Ⅱ型天然澄清剂用于桑叶提取液的澄清工艺  

Clarification Process of Mulberry Leaf Extracts with ZTC1+1Ⅱ Natural Clarifying Agent

作　　者：周杏子[1,2] 贺胜[2] 何海[1,2] 吴新荣[2] 

机构地区：[1]华南理工大学轻工与食品学院,广州510640 [2]广州军区广州总医院药学部,广州510010

出　　处：《医药导报》2016年第5期505-509,共5页Herald of Medicine

基　　金：广东省重大科技专项(2011A080300004)

摘　　要：目的优化ZTC1+1Ⅱ型天然澄清剂用于桑叶提取液除杂的工艺。方法以桑叶生物碱保留率、黄酮保留率、多糖保留率和固形物去除率为指标,进行单因素实验,分别考察澄清剂加入顺序、澄清剂加入量、药物浓度、作用温度、搅拌时间、搅拌速率及水浴保温时间对除杂效果的影响。在单因素实验的基础上,应用效应面分析法优化澄清工艺条件。结果 ZTC1+1Ⅱ型天然澄清剂2组分加入顺序为先B组分后A组分,澄清剂B组分加入量为10%药液体积,A组分为5%,药物浓度为0.17 g·m L-1,作用温度为76℃,搅拌速率120 r·min-1,搅拌10 min,80℃水浴保温30 min,静置12 h的工艺条件下,桑叶生物碱保留率为92.5%,桑叶黄酮保留率为90.2%,桑叶多糖保留率为91.1%,固形物去除率为26.5%。结论 ZTC1+1Ⅱ型天然澄清剂能够有效纯化桑叶提取液,且基于效应面法优化的工艺条件,方法简单可行,澄清度好。Objective To determine the optimum process of ZTC1＋1Ⅱnatural clarifying agents edulcorated mulberry leaf extracts. Methods The remaining rate of mulberry leaf alkaloid,flavone,polysaccharide and the solids removal rate were used as indexes,optimized by single factor experiment to study the effect of some factors,such as the order and dosage of clarifying agents,the concentration and the temperature of the extracting solution,whisking time,whisking speed and holding time,response surface methodology was selected to decide the best clarfication process. Results The optimal process：the order of the ZTC1＋1Ⅱ natural clarifying agents was part B after part A,the dosage of clarifiers B and A were 10% and 5%volume of the extract,the extract solution was 0.17 g·mL^-1 ,the temperature was 76 ℃,the whisking speed was 120 r·min^-1 , the whisking time was 10 min,80 ℃ holding 30 min,and standing time was 12 h,the remaining rate of mulberry leaf alkaloid, flavone and polysaccharide were 92.5%,90.2% and 91.1%,the solids removal rate was 26.5%. Conclusion ZTC1＋1Ⅱnatural clarifying agents could effectively purify the extracts of mulbery leaf,optimized by the response surface method,the method is simple and feasible,and clarity is good.

关 键 词：桑叶 澄清剂 ZTC1+1Ⅱ型 生物碱 黄酮 多糖 

分 类 号：R282.71[医药卫生—中药学] TQ460.6[医药卫生—中医学]