玉米茎秆相关性状多效性QTL检测及相应R程序包编制  

Pleiotropic QTL detection for stalk traits in maize and related R package programming

作　　者：李俊辉[1] 胡海晓[2] 孟玉杰[1] 程鲲[1] 李国梁[1] 刘文欣[1] 陈绍江[1] 

机构地区：[1]中国农业大学国家玉米改良中心,北京100193 [2]霍恩海姆大学植物育种和种子科学及群体遗传学研究所,德国斯图加特70599

出　　处：《中国农业大学学报》2016年第6期9-18,共10页Journal of China Agricultural University

基　　金：国家玉米产业技术体系(CARS-02-09);教育部留学回国人员启动项目

摘　　要：利用回归分析、似然比检验、排列检验、参数自助检验和交叉验证等统计分析方法,构建了多系杂交群体多性状QTL定位模型,并编制了配套的R语言程序包,该套模型和R程序包同样适用于两系杂交群体QTL检测和多效性QTL分析。基于本研究的模型和R程序包,以玉米自交系B73和高油突变体Ce03005为亲本产生的216个RIL群体为研究材料,对玉米茎秆相关性状进行多效性QTL检测分析,结果表明:Bin5.03区域内调控最大折断力矩MF和节间直径d的QTL,Bin6.01区域内调控单位体积中性洗涤纤维NDF和节间含水量Wwc的QTL,Bin8.03区域内影响节间干重WD和MF的QTL均为多效性QTL;在5号染色体上,调控d和NDF的QTL与调控MF和NDF的QTL均为连锁的QTL。本研究不仅为玉米茎秆性状的遗传改良提供了参考,也为其他作物遗传育种研究过程中多效性QTL检测提供了软件分析平台。Determination of pleiotropism with QTL linkage benefits multiple traits improvement in plant breeding.A prototype of pleiotropic QTL detection for multiple-line crossed population was built basing on regression analysis,approximate likelihood ratio test,permutation test and parametric bootstrap.And the prototype is also suitable to biparental population.The prototype was used to map and determinate pleiotropic QTL with linked QTL for multiple traits of maize stalk in a RIL population constructed from inbred line B73 and Ce03005.All genotypes were evaluated at two locations in two years totally four macro environments for phenotypic traits.The results are shown as follows：There is one pleiotropic QTL on chromosome 5controlling traits of moment of maximum bending force（MF）and internode diameter（d）;QTLs affecting neutral detergent fiber of unit volume（NDF）and internode water content（Wwc）at bin6.01 and QTLs affecting Internode dry weight（WD）and MF at bin8.03 are also pleiotropic QTLs;However,on chromosome 5,QTLs controlling traits of d and NDF,and QTLs for traits of MF and NDF are linked QTLs but not pleiotropic QTLs.The QTLs determination is valuable to maize breeding and the analysis prototype is useful to other crop species.

关 键 词：玉米 茎秆 紧密连锁QTL 多性状 多效性QTL 

分 类 号：O212.4[理学—概率论与数理统计] S513[理学—数学]