^(60)Co-γ诱变大豆品质与农艺性状关联度分析  

Correlation analysis between quality and agronomic traits of mutation soybean irradiated by ^(60)Co-γ

作　　者：郭数进[1] 杨凯敏[1] 周永航[1] 王燕平[2] 李贵全[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]黑龙江省农业科学院牡丹江分院,黑龙江牡丹江157041

出　　处：《中国农业大学学报》2016年第6期19-27,共9页Journal of China Agricultural University

基　　金：山西省农业科技成果转化资金项目;山西农业大学科技创新基金项目(2006057)

摘　　要：以经^(60)Co-γ辐射的大豆品种晋大78M5代为材料,应用灰色关联度分析诱变后代蛋白质、脂肪含量与各农艺性状的关联度;利用聚丙烯酰胺凝胶电泳(SDS-PAGE)技术,研究后代各品系7S、11S及其亚基相对含量,并分析其与蛋白质、脂肪含量的相关性。结果表明:各性状中,脂肪和蛋白质的变异系数最小,主茎粗、株高、百粒重和主茎节数4个农艺性状的变异程度较低;单株产量、主茎节数、百粒重和主茎粗4个农艺性状与蛋白质含量、脂肪含量均有较高的关联度;7S相对含量与11S相对含量呈极显著负相关,7S、11S各亚基与其相对含量均呈极显著正相关;7S相对含量与蛋白质、脂肪含量均呈正相关,11S相对含量与蛋白质含量呈正相关,与脂肪含量呈负相关。品系14、16的α′、α亚基相对含量较低,可用于特殊加工,也可为选育7S球蛋白缺失品种提供种质资源。Taking soybean cultivar Jinda78 and its mutation progeny M5 irradiated by 60Coγas materials,coefficient of variation was utilized for genetic analysis of quality and 11 agronomic traits in mutation progeny.Grey relational analysis was conducted for correlation between quality and 11 agronomic traits in mutation progeny.SDS-PAGE was used for analysis of relative contents ofβ-conglycinin soy protein（7S）,glycinin soy protein（11S）,and their subunits.Furthermore,the correlation between protein and oil contents and the relative contents in mutation progeny were researched.The results showed that：Among the traits,oil and protein displayed the lowest coefficients of variation;Mainstem thickness,plant height,100-seed weight and mainstem node number showed lower variation degree;Yield per plant,mainstem node number,100-seed weight and mainstem thickness had higher relational degree with the contents of both protein and oil;The relative content of 7Sshowed significant and strong negative correlation with the relative content of 11 S.All relative contents of the subunits of 7Sand 11 Sshowed significant and strong positive correlation with their relative contents;The relative content of 7Shad positive correlation with both protein and oil contents.The relative content of 11 Shad positive correlation with protein content,and negative correlation with oil content.In conclusion,line14 and 16of Jinda78M5 had lower relative contents of the subunits ofα′andα.Therefore,these two lines can be used in special processing and plant breeding for cultivars withoutβ-conglycinin soy protein.

关 键 词：大豆 60Co-γ诱变 品质 农艺性状 关联度 

分 类 号：S565.1[农业科学—作物学]