双价抗寒基因CBF3/COR15A转化大白菜的初步研究  

Preliminary research of the transformation of bivalent cold-resistance genes CBF3/COR15A into Chinese cabbage

作　　者：孟平红[1] 李桂莲[1] 李苍山[2] 蔡霞[1] 郭惊涛[1] 邓英[1] 张兴国[3] 

机构地区：[1]贵州省园艺研究所,贵阳550006 [2]四川省巴中市第二中学,四川巴中636600 [3]西南大学园艺园林学院,重庆400715

出　　处：《中国农业大学学报》2016年第6期41-51,共11页Journal of China Agricultural University

基　　金：贵州省优秀科技教育人才省长专项资金项目(黔省专合字[2009]61号);贵州省农科院人才启动项目(黔农科院[2009]002号);国家自然科学基金资助项目(31160238);国家星火计划重大项目(2012GA820001);贵州省科技重大专项(黔科合重大专项字[2011]6001及重大专项字[2013]6061-1);贵州省重点攻关(黔科合成转字[2015]5022A号)

摘　　要：为探索拟南芥的冷诱导转录因子CBF3和抗寒基因COR15A在"黔白"系列大白菜的转化体系,以"黔白"系列大白菜3个优良自交系作为研究对象,分别用大白菜4d苗龄的带柄子叶、半子叶、下胚轴作为外植体,利用农杆菌介导的方法将同时含有CBF3和COR15A双价抗寒基因的表达载体转入大白菜,就影响大白菜再生和遗传转化的因素进行研究。通过抗生素筛选得到大白菜T0代转基因抗性植株36株,经PCR检测后获得转基因阳性植株6株。经抗寒性初步鉴定,转基因大白菜植株抗寒性超过对照。To establish＂Qianbai＂series cabbage regeneration-transforming technology system of cold resistance gene CBF3 and COR15 A,＂Qianbai＂series 3inbred lines of Chinese cabbage were used as the research object.Using the cotyledons of petiole,half leaf,hypocotyl of 4dage seedlings of Chinese cabbage as explants,and the cotyledon petiole of Zui4 as the receptor of genetic transformation,we transferred the expression vector with bivalent cold resistance gene CBF3 and COR15 Ainto Chinese cabbage by agrobacterium-mediated method.Factors affecting explants regeneration and genetic transformation of Chinese cabbage were also studied.By antibiotic selection,we obtained 6transgenic plants by PCR from 36T0 transgenic plants.Preliminary identification of cold resistance showed that the cold resistance of transgenic cabbage plants is higher than that of control plants.

关 键 词：大白菜 遗传转化 抗寒基因CBF3 抗寒基因COR15A 

分 类 号：S634.1[农业科学—蔬菜学]