增液汤中梓醇含量测定  

Content of Catalpol in Zengye Tang by High Performance Liquid Chromatography

作　　者：张巍[1] 石延榜[2] 段卫娜[2] 吴若男 

机构地区：[1]河南中医学院第三附属医院,河南郑州450008 [2]河南中医学院,河南郑州450046

出　　处：《中医学报》2016年第4期570-573,共4页Acta Chinese Medicine

基　　金：2015年国家中医药行业科研专项(201507002);国家中医药行业科研专项基金(201107007)

摘　　要：目的:建立增液汤中梓醇含量测定方法,进行增液汤方剂质量标准研究。方法:采用高校液相色谱法,色谱柱Aichrom Bond-AQ C_(18)(4.6 mm×250 mm,5μm);以乙腈-(体积分数)0.1%磷酸水(1∶99)为流动相;检测波长为210 nm;检测温度为25℃;流速为0.7 m L·min^(-1);理论塔板数以梓醇峰计不得低于1 500。结果:此方法梓醇对照品在0.208~2.080μg线性关系良好,回归方程为:Y=236 445X^(-1)0 670,R^2=1.000 0。3批样品含量测定的结果,增液汤方剂中梓醇的含量均不少于0.80 mg·g^(-1)。结论:增液汤方剂中梓醇含量稳定,可能是其降低血糖的有效成分。Objective：To establish a determination method of catalpol content in Zengye Tang,for the quality standard research of it. Methods：High performance liquid hromatography;Aichrom Bond-AQ C18（4. 6 mm × 250 mm,5 μm） was used and the mobile phase was cetonitrile- 0. 1% phosphori-cacid（ 1 ∶99）; The wavelength of detector was 210 nm; the column temperature was25 ℃ and the flow rate was 0. 7 m L·min-1;Theoretical plate of catalpa alcohol meter peak is not less than 1500. Results：Catalpol showed a good linearity at the range of 0. 208- 2. 080 μg. The regression equation was Y = 236445X-10670,R2= 1. 000 0.Results for the determination of three batch sample content,the content of catalpol in Zengye Tang are not less than 0. 80mg·g-1. Conclusion：The catalpol in the Zengye Tang prescription is stable contents,maybe is the active components of lowering blood sugar.

关 键 词：增液汤 梓醇 高效液相色谱法 质量标准 

分 类 号：R284.2[医药卫生—中药学]