丹参多酚酸盐对ApoE^(-/-)小鼠主动脉壁细胞清道夫受体A和CD36 mRNA表达的影响  

The effects of salvianolate on ApoE-knockout mice's aortic wall cell SR-A and CD36 mRNA expression

作　　者：蔡学杰[1] 高玉琪[2] 李晓燕[2] 陈英剑[3] 梁春[4] 吴宗贵[4] 

机构地区：[1]解放军456医院,山东济南250031 [2]济南军区总医院心内科 [3]济南军区总医院实验诊断科 [4]第二军医大学长征医院心内科,上海200003

出　　处：《实用医药杂志》2016年第5期427-430,共4页Practical Journal of Medicine & Pharmacy

摘　　要：目的观察中药丹参多酚酸盐对ApoE基因敲除小鼠主动脉壁血管细胞清道夫受体A和CD36mRNA表达的影响,探讨丹参多酚酸盐抗动脉粥样硬化进展的作用机制。方法 30只AopE基因敲除小鼠(4周龄,雄性)随机分成4组,即模型组(腹腔注射生理盐水,7只)、丹参多酚酸盐60 mg/kg组(7只)、丹参多酚酸盐120 mg/kg组(8只)、丹参多酚酸盐240 mg/kg组(8只),正常对照组(C57BL/6J野生型小鼠)6只。高脂饮食喂养于24周末时处死各组动物,取主动脉根部连续切片,常规苏木精-伊红染色,观察血管壁形态和脂质沉积情况,采用逆转录聚合酶链式反应对主动脉血管壁细胞清道夫受体A和CD36 mRNA表达进行定性和定量分析。结果 ApoE基因敲除鼠动脉粥样硬化斑块内均可见明显的SR-A和CD36mRNA表达,C57BL/6J小鼠正常血管内仅见少量表达;丹参多酚酸盐(240 mg/kg组)干预后,与模型组比较,ApoE基因敲除鼠动脉粥样硬化斑块内CD36 mRNA表达明显减少,差异有统计学意义(P<0.05),而SR-A mRNA的表达未见明显减少(P>0.05)。结论中药丹参多酚酸盐抗ApoE^(-/-)鼠主动脉粥样硬化可能与其下调CD36mRNA表达,减少巨噬细胞泡沫细胞的形成有关,而与SR-A无关。Objective To observe the effects of salvianolate on Apo E-knockout mice＇s aortic wall cell SR-A and CD36 mRNA expression,and to explore the mechanism of salvianolate＇s anti-atheroselerotic progress. Methods The 30 AopE knockout mice （4 weeks old,male） were randomly divided into 4 groups,namely,the model group （normal saline,Tmice）, salvianolate 60 mg/kg group （7 mice）, salvianolate 120 mg/kg group （8 mice）, salvianolate 240 mg/kg group （8 mice）,normal control group （C57BL/6J wild-type mice,6）. The each group of mice were killed after 24 weeks＂ high-fat diet. The aortic root serial sections were taken for routine hematoxy iin- eosin staining and observing vessel wall morphology and lipid deposition;reverse transcription polymerase chain reaction was used to analyse scavenger receptor A and CD36 mRNA expression on the aorta vascular wall cell in qualitative and quantitative analysis. Results The clear expression of SR-A and CD36mRNA in ApoE knockout mice atherosclerotic plaques were visible,in C57BL/6J mice with normal blood vessels only a small amount of expression was seen;After the intervention of Salvianolate （240 mg/kg group）,CD36 mRNA expression in ApoE knockout mice^s atherosclerotie plaque was significantly reduced compared with model group. The difference was statistically significant（P〈0.05）,while there was no significant decrease in the SR no-A mRNA expression（P〉0.05）. Conclusion The anti-atheroselerosis function of salvianolate may be associated with decreasing CD36mRNA expression and reducing macrophage foam cell formation,but not with SR-A.

关 键 词：丹参多酚酸盐 APOE基因敲除小鼠 清道夫受体 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]