异氟醚对人类胚胎干细胞自我更新能力及分化的影响  

EFFECTS OF ISOFLURANE ON SELF-RENEWAL AND DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS

作　　者：刘怡[1] 殷积慧[1] 王勇[2] 冀翔宇[1] 刘英志[1] 孙敏[3] 

机构地区：[1]青岛大学附属医院麻醉科,山东青岛266071 [2]青岛大学附属医院关节外科 [3]滨州医学院附属医院麻醉科

出　　处：《齐鲁医学杂志》2016年第2期184-187,共4页Medical Journal of Qilu

摘　　要：目的探讨异氟醚对人类胚胎干细胞（hESCs）自我更新能力及分化的影响。方法将传代后1d的hESCs随机分为实验组和对照组,实验组细胞暴露于体积分数0.02异氟醚、体积分数0.05CO_2和体积分数0.21O_2的混合气体中6h,对照组细胞暴露于体积分数0.05CO_2和体积分数0.21O_2的混合气体中6h。观察hESCs的生长情况,并检测两组细胞干性标志性基因（Nanog、Sox2、Oct4、Lin28）及各胚层标志性基因（AFP、T、Nestin）的mRNA水平。结果传代后5d,实验组细胞数目明显低于对照组（t=3.40,P〈0.01）;实验组细胞干性基因的mRNA水平明显低于对照组,各胚层标志性基因的mRNA水平均高于对照组（t=2.67~3.89,P〈0.05）。结论异氟醚可以抑制hESCs的自我更新能力,并促进其提前分化。Objective To explore the effects of isoflurane on self-renewal and differentiation of human embryonic stem ceils （ESCs）. Methods Human ESCs--after one day of passage--were randomized to two groups as experiment group and con- trol group. The ceils in the experiment group were exposed to 2% isoflurane, 5% CO2, and 21% 02 for 6 h, and that in the con- trol group were exposed to 5% CO2 and 21% 02 for 6 h. The growth of ESCs in the two groups were observed, and the mRNA levels of sternness markers--Nanog, Sox2, Oct4, Lin28--and marker genes of three germ layers of ESCs--AFP,T and Nestin-- were detected. Results On day 5 of the passage, the number of human ESCs in the experiment groups was significantly lower than that in the control group （t = 3.40, P〈0.01）. The mRNA levels of sternness markers in experiment group were lower than that in the control group and the mRNA levels of marker-genes of three germ layers were higher （t = 2.67- 3.33, P % 0.05）. Conclusion Isoflurane can inhibit the self-renewal capacity of human embryonic stem cells and motivate them to he differentiated in advance.

关 键 词：异氟醚 胚胎干细胞 细胞增殖 细胞分化 

分 类 号：R971.2[医药卫生—药品] R329.24[医药卫生—药学]