黄芩素对HCMV感染人神经干细胞的作用及机制  

BAICALEIN ON HUMAN NEURAL STEM CELLS INFECTED BY HCMV:IT'S EFFECTS AND MECHANISM

作　　者：魏艳[1] 李玲[1] 于向民[1] 徐敬国[1] 王斌[2] 钱冬萌[2] 

机构地区：[1]青岛大学医学院组织胚胎学教研室,山东青岛266071 [2]青岛大学医学院病原微生物学教研室,山东青岛266071

出　　处：《青岛大学医学院学报》2016年第2期160-163,168,共5页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金资助项目(81070501);青岛市博士后应用研究项目(2015153)

摘　　要：目的探讨黄芩素（BAI）对人巨细胞病毒（HCMV）体外感染的人神经干细胞的作用及其可能机制。方法体外培养人神经干细胞,给予HCMV感染和不同浓度的BAI处理24、48、72h,观察其形态学变化,采用MTT法检测BAI对HCMV感染人神经干细胞增殖的影响,采用实时荧光定量PCR技术检测BAI对HCMV感染人神经干细胞中miR-200和miR-UL112-3p表达的影响。结果在病毒感染后24h,HCMV组细胞出现明显的细胞病变效应（CPE）,10μmol/L BAI＋HCMV组细胞CPE不明显。HCMV感染后24、48、72h,10μmol/L BAI＋HCMV组细胞MTT检测吸光度值均高于HCMV组（F=18.61-22.57,P〈0.05）。HCMV感染人神经干细胞后24、48、72h,miR-200和miR-UL112-3p表达下调,10μmol/L BAI＋HCMV组和HCMV＋10μmol/L BAI组细胞miR-200和miR-UL112-3p表达明显高于HCMV组（F=2.37~19.26,P〈0.05）。结论一定浓度BAI能够抑制HCMV感染人神经干细胞的增殖,这可能与其上调细胞内miR-200和miR-UL112-3p的表达有关。Objective To study the protective effects of baicalein （BAD on neural stem cells infected by HCMV in vitro, and its possible mechanisms. Methods Human neural stem cells were cultured in vitro and infected by HCMV. The infected cells were then treated with different concentrations of BAI for 24, 48 and 72 h, their morphologic variations were observed. Ap- plying MTT assay, the impact of BAI on HCMV-infected human neural stem cell proliferation was assessed. The expressions of miR-200 and viral coded miR-UL112 3p in the infected cells were detected using real-time PCR. Results At 24 hours after infec tion, HCMV group showed obvious cytopathic effect （CPE）, while no obvious CPE effect was found in 10 μmol/L BAI-b HCMV group. After 24, 48 and 72 hours of the infection, MTT results showed that the absorbance values in 10 μmol/L BAI＋HCMV group were all higher at 24, 48, and 72 hours than HCMV group （F=lS.61-22.S7,P〈0.05）, the expressions of miR-200 and miR-UL112-3p were down-regulated in HCMV group, and that in 10 μmol/L BAI-HCMV group were higher than that in HCMV group （F=2.37-19.26,P〈0.05）. Conclusion A certain concentration of Baicalein can inhibit the proliferation of human neural stem cells infected by HCMV, which might be related to up-regulating the expressions of miR-200 and miR UL112-3p.

关 键 词：黄芩素 巨细胞病毒 神经干细胞 微RNAS 

分 类 号：R285[医药卫生—中药学]