海藻酸钙纤维制备新型医用止血敷料的评价研究  

A NEW TYPE MEDICAL HEMOSTATIC DRESSING PREPARED BY ALGINATE FIBER:EVALUATION STUDIES

作　　者：初晓夏 王斌[1] 王海涛[1] 陈豪[1] 崔飞艳 

机构地区：[1]青岛大学医学院病原生物学教研室,山东青岛266071

出　　处：《青岛大学医学院学报》2016年第2期178-181,共4页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省科技发展基金项目(2013GGX10215)

摘　　要：目的与纱布、明胶海绵止血敷料比较,评价海藻酸钙纤维止血敷料的止血效果、生物相容性以及抗菌性能。方法将3种止血敷料敷于新西兰兔背部两侧创口,记录出血时间,用氰化高铁血红蛋白(Hb)检测试剂检测Hb吸光度值评价止血效果,采用溶血实验、急性全身毒性实验、体外细胞毒性实验评价敷料生物相容性,采用振荡法实验评价抗菌性能。结果海藻酸钙纤维、纱布、明胶海绵组的出血时间分别为(95.28±3.95)、(138.33±5.98)、(101.25±4.53)s,差异有显著性(F=2 433.233,P<0.05);Hb吸光度值分别为1.269±0.011、1.146±0.013、1.754±0.013,差异有显著性(F=24 764.804,P<0.05),出血量分别为(0.921±0.021)、(0.900±0.012)、(1.607±0.028)g,差异亦有显著性(F=3 364.499,P<0.05)。海藻酸钙纤维细胞毒性为1级,溶血率为0.8%,抗菌率为49%。结论海藻酸钙纤维敷料止血速度快、止血效果好,可有效控制出血量;细胞毒性小,急性全身毒性反应及溶血率低,有良好的生物相容性;有一定的抗菌作用。Objective To assess the hemostatic effect, biocompatibility and antibacterial property of calcium alginate fi her hemostatic dressings （CAFHD） by comparing with hemostatic dressings such as gauze and gelatin sponge. Methods Three kinds of hemostatic dressings-CAFHG, gause and gelatin sponge-were applied on bilateral wounds of the back of New Zealand rabbit. Bleeding time was recorded. Employing cyanmethemoglobin detection reagent, the absorbance of hemoglobin （Hb） was deter- mined to assess the hemostatic effect. The biocompatibility was assessed using hemolysis test, acute general toxicity test and in vitro cytotoxicity test. Antibacterial property was evaluated using oscillation experiment. Results The bleeding time in alginate fiber, gauze and gelatin sponge was （95.28±3.95）, （138.33±5.98） and （101.25±4.53）, respectively （F=2 433.233,P〈0.05）. The absorbance values of Hb were 1.269±0.011,1.146±0.013, and 1.754±0.013 （F=24 764.804,P〈0.05）; the amount of bleeding was （0.921±0.021）, （0.900±0.012） and （1.607±0.028） g （F=3 364.499,P〈0.05）. Cytotoxicity of seaweed fiber was grade 1, hemolytic rate was 0.8%, and antibacterial rate was 49%. Conclusion Alginate dressings are effective for immediate stopping bleeding and controlling blood loss, with little cytotoxicity, acute general toxic reactions, low hemolysis rate, favourable biocompatibility, and a certain antibacterial action.

关 键 词：海藻 生物敷料 止血 组织相容性 细胞毒性 抗生作用 评价研究 

分 类 号：R318.08[医药卫生—生物医学工程]