栽培年限对东北人参中皂苷合成基因表达量的影响  

The effect of cultivation years on saponin synthetic gene expression quantity in northeast Panax ginseng

作　　者：林枫 刘佳[2] 陈华峰[2] 

机构地区：[1]辽宁省林业调查规划院,辽宁沈阳110122 [2]森林植物生态学教育部重点试验室(东北林业大学),黑龙江哈尔滨150040

出　　处：《吉林林业科技》2016年第3期19-22,共4页Journal of Jilin Forestry Science and Technology

基　　金：国家自然科学基金青年科学基金项目[1400337]

摘　　要：以不同栽培年限的东北人参为材料,对其皂苷生物合成途径中各关键酶基因表达量进行研究,结果表明:SS、SQE1、SQE2、β-AS和CAS基因表达量在2 a生时均达到峰值,之后呈现下降趋势。在相关性分析中,SS、SQE1、β-AS和CAS四种基因表达量呈显著正相关(p<0.05);SS基因和SQE1基因、SS基因和CAS基因、SQE1基因和CAS基因的表达量呈极显著正相关(p<0.01);而β-AS、CAS和SQE2三种基因的相关性亦达到显著水平(p<0.05)。The each key enzyme gene expression quantity in the biosynthetic pathway of saponin was researched by choosing northeast Panax ginseng of different cultivation years as material, the result showed that the gene expression quantity of SS, SQE1 ,SQE2,β- AS and CAS reached the peak when it was 2 years and then presented downtrend. The gene expression quantity of SS ,SQE1,β- AS and CAS presented significant positive eorrelation（p 〈 0.05 ）in correlation analysis. Furthermore, the expression quantity of SS gene and SQE1 gene,SS gene and CAS gene,SQE1 gene and CAS gene presented extreme significant positive correlation（p 〈 0.01 ）. The correlation of β-AS ,CAS and SQE2 also reached significance level （p 〈0. 05）.

关 键 词：栽培年限 东北人参 皂苷 生物合成 基因表达 

分 类 号：S567.51[农业科学—中草药栽培]