2007-2014年柯萨奇病毒B5四川分离株的基因特征分析  

Genetic characteristics of Coxsackie virus B5 isolates in Sichuan province over 2007-2014

作　　者：马小珍[1] 童文彬[1] 刘李[1] 曹冉冉[1] 陈娜[1] 

机构地区：[1]四川省疾病预防控制中心,成都610041

出　　处：《中华实验和临床病毒学杂志》2016年第2期157-160,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 了解2007-2014年四川省急性弛缓性麻痹（acute flaccid paralysis,AFP）病例中柯萨奇病毒B组5型（Coxsackie virus B5,CVB5）的基因特征.方法 对四川省2007 ～2014年AFP病例中分离到的10株CVB5进行全VP1区逆转录-聚合酶链（RT-PCR）反应扩增和核苷酸序列测定,构建遗传进化树进行基因特征分析.结果 10株分离株均为D基因型,与CVB5原型株（Faulkner株）之间的核苷酸同源性和氨基酸同源性分别为80.4％ ～ 81.9％和95％ ～97.1％.2014年1株资阳分离株和1株成都分离株VP1区氨基酸序列完全一致,1株南充分离株和1株宜宾分离株VP1区核苷酸和氨基酸同源性均为100％.结论 2007-2014年四川地区AFP病例中分离到的CVB5为D基因型,且遗传特性较为稳定.Objective To investigate genetic characteristics of Coxsackie virus B5 （CVBS） in acute flaccid paralysis（AFP） cases in Sichuan Province.Methods 10 CVB5 strains isolated from stool samples of AFP cases in Sichuan Province over 2007-2014 were subjected to entire VP1 coding region amplification by reverse transcription-polymerase chain reaction （RT-PCR） and nucleotide sequencing,and phylogenetic tree was constructed for genetic characterization.Results All of the 10 strains were identified as genogroup D.The nucleotide and the amino acid homologies were 80.4%-81.9% and 95%-97.1%,which compared with the Faulkner prototype strain.The amino acid homologies between Ziyang strain and Chengdu strain obtained in 2014 were 100%,respectively.The nucleotide and the amino acid homologies between Nanchong isolate and Yibin isolate obtained in 2014 were both 100%,respectively.Conclusions The isolates from AFP cases in Sichuan Province over 2007-2014 were belong to genogroup D.The genetic characteristics of 10 strains were stable.

关 键 词：急性驰缓性麻痹 柯萨奇病毒B组5型 基因特征分析 

分 类 号：R512.5[医药卫生—内科学] R440[医药卫生—临床医学]