九节菖蒲中的酚酸类化合物  

Phenolic Acid Compounds from Anemone altaicae Rhizoma

作　　者：王彦志[1] 刘阳[1] 赵会丽[1] 曾光[1] 张萌[1] 

机构地区：[1]河南中医学院呼吸疾病诊疗与新药研发河南省协同创新中心,郑州450046

出　　处：《中国药学杂志》2016年第10期798-801,共4页Chinese Pharmaceutical Journal

基　　金：河南中医学院科技创新团队;科技创新人才支持计划资助项目(2015XCXTD02);呼吸疾病诊疗与新药研发河南省协同创新中心研究生科研创新基金资助项目(教科技[2013]638号)

摘　　要：目的研究九节菖蒲（Anemone altaica Fisch ex C.A.Mey）中的酚酸类化学成分。方法采用Diaion HP-20大孔吸附树脂,Sephadex LH-20凝胶,硅胶等柱色谱技术并结合制备液相对九节菖蒲水溶性成分进行分离纯化,通过理化性质和波谱数据结合文献对化合物结构进行鉴定,对分离得到的新化合物来源进行了推测,同时分析了酚酸产物的生物合成来源。结果从九节菖蒲水溶性部位分离并鉴定了11个化合物：阿魏酰酒石酸甲酯A（Ⅰ）,阿魏酰酒石酸甲酯B（Ⅱ）,阿魏酰酒石酸乙酯B（Ⅲ）,阿魏酰乳酸单甲酯（Ⅳ）,阿魏酰乳酸二甲酯（Ⅴ）,香草酸葡萄糖苷（Ⅵ）,对羟基苯甲酸葡萄糖苷（Ⅶ）,绿原酸甲酯（Ⅷ）,3-O-阿魏酰-奎宁酸（Ⅸ）,5-O-feruloyl-3-O-（β-D-glucopyranosyl）-2-deoxy-D-ribono-γ-lactone（Ⅹ）,carboxymethyl isoferulate（Ⅺ）。结论化合物Ⅰ-Ⅴ为新化合物,推测其为人工产物,化合物Ⅵ-Ⅺ为从该植物首次分离得到。OBJECTIVE To study the chemical constituents in the roots of Anemone altaica. METHODS The isolation and purification of the compounds were performed by chromatography on Diaion HP-20,Sephadex LH-20,and silica gel,combined with preparation liquid chromatography. Their structures were determined by comparison of their physicochemical characteristics and spectral data with literatures. RESULTS Eleven compounds were obtained,and their structures were identified as methyl feruloyl-tartarate A（ Ⅰ）,methyl feruloyl-tartarate B（ Ⅱ）,ethyl feruloyl-tartarate B（ Ⅲ）,mono-methyl feruloyl-lactate（ Ⅳ）,dimethyl feruloyl-lactate（ Ⅴ）,vanillic acid-4-O-β-D-glucopyranoside（ Ⅵ）,p-hydroxybenzoic acid glucoside（ Ⅶ）,methyl chlorogenate（ Ⅷ）,3-O-fernloylquini acid（ Ⅸ）,5-O-feruloyl-3-O-（ β-D-glucopyranosyl）-2-deoxy-D-ribono-γ-lactone（ Ⅹ）,and carboxymethyl isoferulate（ Ⅺ）.CONCLUSION Compounds Ⅰ- Ⅴ are new compounds,and are speculated to be a man-made products. Compounds Ⅵ- Ⅺ are isolated from the plants of anemone genus for the first time.

关 键 词：毛茛科 阿尔泰银莲花 阿魏酰酒石酸甲酯 绿原酸甲酯 生物合成途径 

分 类 号：R284[医药卫生—中药学]