肾虚质大鼠学习记忆能力与海马NMDA受体表达的实验研究  

Learning and Memory Capacity and NMDA Receptor Expression in Shen Deficiency Constitution Rats

作　　者：孙瑜嬬[1] 孙耀光[1] 张琪[1] 王晓棣[2] 王兴[2] 孙理军[1] 

机构地区：[1]陕西中医药大学基础医学院,陕西712046 [2]陕西中医药大学研究生院,陕西712046

出　　处：《中国中西医结合杂志》2016年第5期597-601,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：陕西省科技厅研究项目(No.2013JM4033);陕西省重点实验室研究项目(No.13JS031)

摘　　要：目的观察肾虚质大鼠学习记忆能力与海马N-甲基-D-天冬氨酸(N-methyl-D-aspartic acid,NMDA)受体表达变化,探讨"肾藏志"中医学理论的物质基础与神经生物学机制。方法将40只SD大鼠随机分为模型组、左归丸、右归丸组及空白组(产自正常孕鼠),每组10只,采用"猫吓鼠"经典造模法制备先天不足加后天失养复合型肾虚质仔鼠模型。对仔鼠恐吓同时开始给药,左归丸、右归丸组分别给予左归丸混悬液0.1875 g/mL、右归丸混悬液0.0938 g/mL灌胃,空白组及模型组给予等量生理盐水灌胃,每日1次,每周用药5天,连续2个月。通过Morris水迷宫实验检测大鼠学习记忆能力,采用免疫组化法检测海马组织NMDA受体亚基NR2A、RN2B蛋白表达水平。结果与空白组比较,模型组Morris水迷宫实验潜伏期及总路程增加(P<0.05);与模型组比较,左归丸及右归丸组上述指标均降低(P<0.05)。与空白组比较,模型组海马组织NMDA受体亚基NR2A、NR2B蛋白表达明显降低(P<0.05);与模型组比较,左归丸、右归丸组NR2A及NR2B蛋白表达明显升高(P<0.05)。结论肾虚质大鼠学习记忆能力退化且海马NMDA受体表达低下,补肾药物左归丸、右归丸可上调NMDA受体表达,提高其学习记忆能力,从而改善肾虚质脑功能退化状态。Objective To explore material bases and neurobiological mechanisms of ＂Shen storing will＂ by observing learning and memory capacities and N-methyl-D-aspartic acid（NMDA） receptor expressions in Shen deficiency constitution（SDC） rats. Methods Totally 40 SD rats were randomly divided into the model group, the Zuogui Pil（ZP） group, the Yougui Pil（YP） group, the blank control group（consisting of normal pregnant rats）, 10 in each group. SDC young rat model（inherent deficiency and postnatal malnutrition） was prepared by the classic way of ＂cat scaring rat＂. Medication started when they were scared by cat. Rats in the ZP group and the YP group were administered by gastrogavage with ZP suspension 0. 1875 g/m L and YP suspension 0. 0938 g/m L respectively. Equal volume of normal saline was administered to rats in the blank control group and the model group by gastrogavage. Al medication was given once per day, 5 days in a week for 2 consecutive months. Learning and memory capacities were detected by Morris water maze test. Expressions of NMDA receptor subunits NR2 A and NR2 B in hippocamus were detected by immunohistochemical method. Results Compared with the blank control group, the latency period, total distance in Morris water maze test were longer in the model group（P〈0. 05）. Al the aforesaid indices all decreased in the ZP group and the YP group, with statistical difference when compared with the model group（P〈0. 05）. The protein expressions of NR2 A and NR2 B in hippocamus were lower in the model group than in the blank control group（P〈0. 05）. But when compared with the model group, they were obvi-ously higher in the ZP group and the YP group（P〈0. 05）. Conclusions SDC rats had degenerated learning and memory capacities and lowered NMDA receptor expressions. ZP and YP could up-regulate learning and memory capacities and NMDA receptor expressions, thereby improving deterioration of brain functions in SDC rats.

关 键 词：肾虚质 学习记忆能力 N-甲基-D-天冬氨酸受体 

分 类 号：R259[医药卫生—中西医结合]