SBBR反应器运行中生物膜胞外聚合物的变化特征  

Variations of Extracellular Polymeric Substances of Bio-film in Operation Processes of SBBR

作　　者：李延 解迪[2] 梁文艳[2] 隋丽丽[2] 

机构地区：[1]北京市凉水河管理处,北京100069 [2]北京林业大学环境科学与工程学院,北京100083

出　　处：《环境科学与技术》2016年第4期95-101,共7页Environmental Science & Technology

基　　金：国家水体污染控制与治理技术重大专项(2012ZX07307-001-006;2013ZX07209-001-003)

摘　　要：以进水、静置、曝气和排水作为SBBR反应器的运行周期,研究了在不同溶解氧和换容比条件下运行周期内生物膜EPS的产生与变化特征。扫描电镜（SEM）结果显示,生物膜由大量丝状菌组成,菌胶团和累枝虫交联聚集在其周围,生物膜外层生物相明显比内部丰富。当DO从2～3 mg/L升到3～5 mg/L时,EPS的含量从147.7μg/（mL填料）升至286.0μg/（mL填料）,再升至5～6 mg/L时,EPS稍有降低至271.3μg/（mL填料）。随着换容比从1/3升高至2/3,EPS的含量逐渐从121.5μg/（mL填料）下降到86.2μg/（m L填料）。在运行周期内,进水后处于静置状态1 h的EPS产生量最大,达到148.0μg/（mL填料）,此时EPS中荧光类有机物含量也最高,且以芳香性蛋白质Ⅰ（λEx/λEm（220～250）/（280～330）nm）、芳香性蛋白质Ⅱ（λEx/λEm（220~250）/（330~380）nm）和微生物沥出物（λEx/λEm（250～280）/（290～380）nm））为主。Characteristics of extracellular polymeric substances（EPS） of bio-film were studied using a bench scale SBBR（sequencing batch biofilm reactor）. Investigation of the bio-film characteristics under different process conditions and operation period of the SBBR were conducted with the help of a SEM. The investigation found that the biological membrane was composed of a large number of filamentous bacteria and Epistylis surrounded by zoogloea,while the bio-film organisms of outer layer had more microorganisms than those of inner layer. When DO rose from the ranges of 2～3 mg/L to 3～5 mg/L,EPS content increased from 147.7 to 286 μg/（m Lof carrier）,and then slightly decreased to 271.3 μg/（mL of carrier）as DO rose to the range of 5～6 mg/L. Furthermore,effects of changing volume ratio of the carrier on EPS content were studied;during the running period after the water filling and at the static state for 1 h,EPS reached the maximum value of 148.0 μg/（mL of carrier）,as did the fluorescent organic matters in EPS. The fluorescent organic matters in EPS mainly consisted of aromatic proteins i.e.,protein Ⅰ（λEx/λEm（220～250）/（280～330）nm）,and aromatic protein Ⅱ（λEx/λEm（220～250）/（330～380）nm）,as well as microbial leaching substances（λEx/λEm（250～280）/（290～380）nm））.

关 键 词：胞外聚合物 序批式膜生物反应器 工艺条件 运行周期 污水处理 

分 类 号：X703.1[环境科学与工程—环境工程]