键合型纳米纤维素手性固定相的制备及其手性拆分  

Preparation and chiral separation performance of immobilized nanocellulose chiral stationary phase

作　　者：张晓莉[1,2] 张文娟[3] 王利涛[1] 张霞[1] 董树清[1] 赵亮[1] 师彦平[1] 

机构地区：[1]中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室甘肃省天然药物重点实验室,兰州730000 [2]中国科学院大学,北京100049 [3]中国烟草总公司郑州烟草研究院,郑州450001

出　　处：《分析试验室》2016年第5期497-501,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21405162;21405161);乌鲁木齐市科技局重点项目(Y141320007);阿拉善盟科技局沙产业科研项目资助

摘　　要：通过碱法水解制备了纳米纤维素,并进一步衍生化得到了区域选择性纳米纤维素衍生物,通过化学键合法将纳米纤维素衍生物固定于硅胶基质上,制备了键合型纳米纤维素手性固定相。通过高效液相色谱对纳米纤维素键合的固定相进行手性拆分性能评价,探讨了溶剂效应(醇的浓度、流动相添加剂)对手性拆分的影响。Nanocrystalline cellulose（ NCC） was prepared by alkali hydrolysis process and the regionselectively substituted NCC derivative was synthesized. Then the NCC derivative was chemically bonded to aminopropyl silica gel for the preparation of the immobilized nanocellulose chiral stationary phase（ CSP）. The chiral separation performance of NCC – immobilized CSP was evaluated by high performance liquid chromatography to separate chiral compounds. Moreover,the effects of mobile phase（ concentration of alcohol modifier and mobile phase additive） on chiral separations were investigated. The results showed that chiral compounds were separated on NCC – immobilized CSP with favorable separation performance both in normal phase and reverse phase mode,and this CSP is a promising packing material for the resolution of chiral compounds.

关 键 词：纳米纤维素 键合型手性固定相 手性拆分 高效液相色谱 

分 类 号：O657.7[理学—分析化学]