荧光探针用于定量BSA测定的研究  

Study on the determination of BSA by a fluorescent probe

作　　者：曾晓丹[1] 朱琳[2] 朱宝存[3] 

机构地区：[1]吉林化工学院分析测试中心,吉林132022 [2]吉林化工学院化学与制药工程学院,吉林132022 [3]济南大学资源与环境学院,济南250022

出　　处：《分析试验室》2016年第5期539-541,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然基金项目(21107029);吉林省教育厅资助项目(2014351)资助

摘　　要：设计合成了具有长发射波长（655nin）的荧光探针WZK-31，建立了一种荧光增强分析测定牛血清白蛋白（BSA）的新方法。在模拟人体生理条件下，BSA的加入使探针荧光强度发生不同程度的增强。增强程度与0—53．20μg/mL范围的BSA的质量浓度呈线性关系。方法检出限为0．70μg／mL，干扰小。方法用于样品的测定，加标回收率为89．1％-90．8％。Based on a new fluorescent probe （WZK-31） with long emission wavelength （655 nm）, a new spectrofluorimetric method for determining BSA was developed. In the mimic human physiological conditions, the fluorescence of WZK-31 was enhanced with the addition of BSA. The fluorescence intensity change was linear to BSA concentration in the range of 0-53.20 μg/mL. The results showed that this method had the advantages of simplicity, rapidity, good sensitivity and selectivity, with a detection limit of 0. 70 μg/mL. For the determination of BSA in samples, the recoveries were between 89. 10 and 90.77%.

关 键 词：牛血清白蛋白 荧光光谱法 定量测定 

分 类 号：O657.3[理学—分析化学]