木犀草素在多壁碳纳米管修饰碳糊电极上的电化学行为及其与DNA相互作用  

Electrochemical behavior of luteolin at multi-wall carbon nanotubes modified carbon paste electrode and the interaction of luteolin with DNA

作　　者：尚永辉[1] 刘彬[1] 古元梓[1] 

机构地区：[1]咸阳师范学院化学与化工学院,咸阳712000

出　　处：《分析试验室》2016年第5期549-552,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21475113);陕西省科技计划项目(2012JQ2013);咸阳师范学院'青蓝人才资助项目(XSYQL201507);咸阳师范学院专项科研基金项目(11XSYK104);陕西省电分析化学重点实验室开放基金项目资助

摘　　要：采用循环伏安法、差分脉冲法研究了木犀草素在多壁碳纳米管修饰碳糊电极上的电化学行为。在p H 3.29的B-R缓冲体系中,木犀草素在0.502 V和0.452 V处产生一对明显的氧化还原峰,其峰电流与木犀草素浓度在5.0×10^(-8)~1.0×10^(-5)mol/L范围内呈良好的线性关系,木犀草素在电极上是受吸附控制的2质子、2电子电极反应过程;在此基础上研究了木犀草素与DNA的相互作用,随着DNA的浓度增加木犀草素的电化学信号逐渐降低,表明木犀草素能与DNA发生明显的相互作用,进一步研究表明木犀草素与DNA的相互作用是以嵌入方式为主。Electrochemical behaviors of luteolin at the multi-wall carbon nanotube modified carbon paste electrode were studied by cyclic voltammetry and differential pulse method. It is found experimentally that in p H3. 29 B-R buffer solution,Luteolin ＇s peak potentials are 0. 502 V and 0. 452 V（ vs. SCE） respectively,and the peak currents are linear with the concentration of Luteolin over the range of 5. 0 × 10（- 8） 1. 0 × 10（- 5）mol / L.Besides,the electrode process was double protons-double electrons reaction,which was controlled by absorption.On this basis,the interaction of Luteolin with DNA were studied. The results show that the peak currents of Luteolin gradually decreased with the increased concentration of DNA, indicating the apparent interaction between Luteolin and DNA. Further research shows the interaction between Luteolin and DNA is mainly attributed to the embed mode.

关 键 词：木犀草素 多壁碳纳米管 修饰碳糊电极 相互作用 

分 类 号：TQ460.7[化学工程—制药化工]