石仙桃中总黄酮的提取方法的比较研究  

A Comparative Study of the Extraction Methods of the Total Flavonoids of Pholidota chinensis Lindl.

作　　者：刘建新[1] 余磊[1] 廖芳[1] 李聪[2] 甘小辉 

机构地区：[1]赣南医学院药学院,江西赣州341000 [2]赣南医学院,江西赣州341000

出　　处：《食品研究与开发》2016年第7期62-64,共3页Food Research and Development

基　　金：江西省自然科学基金(2012ZBA20513);赣南医学院产学研项目(201205)

摘　　要：探究石仙桃的总黄酮的提取方法的比较。以乙醇-水为提取体系,分别采用回流法、超声法,探究乙醇浓度、料液比、提取时间等对石仙桃中总黄酮提取的影响,确立最佳条件。通过正交试验证明,超声波辅助提取的最佳工艺为:温度40℃,料液比1∶40(g/mL),提取时间50 min,在此条件下提取的黄酮含量为26.12 mg/g;乙醇回流法提取最佳工艺为:温度60℃,料液比1∶50(g/mL),提取时间50 min,在此条件下提取的黄酮含量为22.03 mg/g。超声波辅助提取法提取石仙桃中总黄酮的提取率高于乙醇回流提取法提取。A comparative study of the extraction methods of the total flavonoids of Pholidota chinensis Lindl.was researched.With ethanol-water as the extraction medium,the reflow,ultrasonic methods were used in the extraction of the total flavonoids of Pholidota chinensis Lindl.. By exploring the ethanol concentration,solid-liquid ratio,extraction time,etc,the optimum extraction conditions were obtained. The optimal extraction conditions of flavonoids by ultrasonic assisted extraction method were extraction time for 50 min,solid to liquid ratio at 1 ∶ 40（ g/mL）,and extraction temperature at 40 ℃.Under the optimum conditions,the content of extracted total flavonoids from Pholidota chinensis Lindl. was 26.12 mg/g.The optimal extraction conditions of flavonoids by ethanol reflux extraction method were extraction time for 50 min,solid to liquid ratio at 1 ∶ 50（ g/mL）,and extraction temperature at 60 ℃.Under the optimum conditions,the content of extracted total flavonoids from Pholidota chinensis Lindl. was 22.03 mg/g. The extraction rate of the total flavonoids of Pholidota chinensis Lindl. by ultrasonic assisted extraction method was higher than by reflux extraction method.

关 键 词：石仙桃 总黄酮 回流提取 超声波辅助提取 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]