一氧化氮合酶抑制剂在实验性兔关节软骨修复中的作用  

Role of nitric-oxide inhibitor SMT in articular cartilage repair in rabbits

作　　者：孙炜[1] 王吉兴[1] 秦立赟[2] 刘晓霞 卢晓[4] 金大地[1] 

机构地区：[1]第一军医大学南方医院骨科,广州510515 [2]解放军188医院外科 [3]第一军医大学消化研究所病理研究室,广州510515 [4]第一军医大学免疫教研室

出　　处：《中华风湿病学杂志》2002年第4期227-230,共4页Chinese Journal of Rheumatology

基　　金：国家自然科学基金资助项目 (3 990 0 1 49)

摘　　要：目的　探讨一氧化氮合酶 (iNOS)抑制剂S 甲基异硫脲在实验性关节软骨修复中的作用。方法　 2 0只新西兰兔随机分为对照组和实验组 ,在双侧股骨髁间滑车关节面作全层软骨缺损。对照组应用纤维蛋白凝胶BMP复合物充填缺损 ;实验组同样充填缺损后 ,并皮下注射iNOS抑制剂S 甲基异硫脲。术后 16周处死动物 ,作修复组织质量评价 ;天狼猩红 苦味酸染色检测Ⅰ型和Ⅱ型胶原分布 ;反转录 多聚酶链反应 (RT PCR)检测iNOS和MMP 9mRNA基因表达 ,BrdU检测软骨细胞增生情况 ,并利用图像仪进行分析。结果　形态学观察证实 ,术后 16周 ,实验组关节软骨缺损修复在评分方面优于对照组 (P <0 0 5 ) ;天狼猩红 苦味酸染色显示实验组Ⅰ型胶原明显少于对照组 ,Ⅱ型胶原多于对照组 ;术后 16周RT PCR在对照组检测到iNOSmRNA和MMP 9mRNA表达 ,实验组仅检测到少量MMP 9mRNA表达。实验组BrdU阳性细胞 8 5个 /m2 明显多于对照组 3 2个 /m2 。结论　iNOS抑制剂SObjective To research the effects of nitric oxide inhibtor on articular cartilage defect repairing.Methods Full thickness defects of cartilage were created in the trochlearn groove of twenty adult New Zealand rabbits.Ten were filled with fibrin glue impregnated with rhBMP as the control,and others were filled with fibrin glue impregnated with rhBMP and hypodermic injection with 5 mg·kg -1 ·12 h -1 S methylisothiourea (SMT).The animals were killed at 16 weeks postoperatively,and the gross appearance of the healed defect was assessed by Wakitani methods.The repair tissue was examined histologically and was evaluated,according to a grading scale.The tissue sections were stained with sirius red against type Ⅰ and,type Ⅱ collagens.The expression of iNOS mRNA and MMP 9 mRNA were examined by RT PCR.The chondrocyte activity was measured by incorporation of BrdU and imaging instrument.Results Sixteenth week after fibrin glue with rhBMP and with hypodermic injection SMT,the damage of anrtilage had a better histological appearance than the control.The histological features that showed improvement were integration within the damage site.The repairing scores were also better for the group treated with SMT than the control ( P <0 05).Sirius red staine demonstrated significantly less type Ⅰ collagen and more type Ⅱ collagen in the defects treated with SMT than in the control.RT PCR showed the MMP 9 mRNA expression in these groups,and iNOS mRNA expression in untreated defects and BMP groups.BrdU showed the SMT could increase positive cell numbers from 3 2/m 2 to 8 5/m 2.Conclusion iNOS inhibitor SMT could decrease NO release,inhibit the MMP 9 mRNA expression,augment chondrocyte proliferation,which could improve the quality of repair tissues.

关 键 词：一氧化氮合酶 酶抑制剂 软骨关节 S-甲基异硫脲 修复 

分 类 号：R687.3[医药卫生—骨科学]