Roux-en-Y胃旁路术对血管内皮细胞P-选择素表达的影响  

Effect of Roux-en-Y Gastric Bypass on the Expression of P- selectin in Vascular Endothelial Cells

作　　者：柴芳[1] 齐凤杰[2] 赵树鹏[1] 王月[2] 王俊[1] 李强[1] 

机构地区：[1]辽宁医学院附属第一医院普外科,辽宁省锦州市121001 [2]辽宁医学院附属第一医院病理科,辽宁省锦州市121001

出　　处：《中国全科医学》2016年第15期1792-1795,共4页Chinese General Practice

基　　金：辽宁省自然科学基金资助项目(2013022044);辽宁医学院博士科研启动基金项目(Y2012B017);辽宁医学院附属第一医院科技启动基金项目(Fyk201211)

摘　　要：目的通过建立2型糖尿病(T2DM)大鼠模型,观察Roux-en-Y胃旁路术(RYGB)对血管内皮细胞P-选择素表达的影响,探讨其对血管内皮细胞的保护机制。方法 2014年10月—2015年4月,采用随机数字表法将60只SD大鼠分为正常对照组(NC组)、T2DM组和RYGB组,各20只。T2DM组和RYGB组予以高脂饮食3周,腹腔注入链脲佐菌素(STZ)30 mg/kg建立T2DM模型;NC组喂养普通饲料,腹腔注入相同浓度的柠檬酸盐缓冲液。RYGB组行RYGB,NC组及T2DM组行假手术。记录大鼠一般情况,包括术前、术后21 d体质量。采用ELISA法检测术前、术后21 d肿瘤坏死因子α(TNF-α)、脂联素水平,HE染色法测定术后21 d血管内膜厚度,免疫组化SABC法检测术后21 d P-选择素表达情况。结果术后RYGB组2只大鼠因吻合口梗阻死亡。T2DM组、RYGB组大鼠术后体质量低于NC组(P<0.05);RYGB组大鼠术后体质量低于T2DM组(P<0.05)。NC组大鼠术后体质量高于术前(P<0.05);RYGB组大鼠术后体质量低于术前(P<0.05)。T2DM组术前、术后TNF-α水平均高于NC组,术前、术后脂联素水平均低于NC组(P<0.05);RYGB组术前TNF-α水平高于NC组,术前脂联素水平低于NC组(P<0.05);RYGB组术后TNF-α水平低于T2DM组,术后脂联素水平高于T2DM组(P<0.05)。RYGB组术后TNF-α水平低于术前,术后脂联素水平高于术前(P<0.05)。3组大鼠血管内膜厚度比较,差异无统计学意义(F=1.337,P=0.271)。NC组、RYGB组P-选择素表达阳性率低于T2DM组(P<0.05)。结论 RYGB可能通过脂联素/TNF-α通路减少P-选择素表达,进而减轻血管内皮细胞损伤程度。Objective To observe the influence of Roux - en - Y gastric bypass（RYGB）on the expression of P -selectin by establishing T2DM rat models and to investigate its protection mechanism for vascular endothelial cells. Methods From October 2014 to April 2015,we divided sixty SD rats into three groups：normal control group（NC group）,T2DM group and RYGB group,with 20 rats in each group. T2DM group and RYGB group were administrated with high fat diet for three weeks and were given 30 mg/ kg streptozotocin（STZ）by intraperitoneal injection to build T2DM models,and NC group was fed with normal fodder and was given citric acid buffer solution of the same concentration. RYGB was undertaken in RYGB group,and sham operation was undertaken in NC group and T2DM group. The general data of the rats were recorded,including body weight before surgery and 21 days after surgery. The levels of TNF-α and adiponectin concentration were detected by ELISA before surgery and 21 days after surgery;vessel intima thickness was determined by HE staining method 21 days after surgery,and the expression of P - selectin was detected by immunohistochemistry SABC method 21 days after surgery. Results Two rats in RYGB group died from anastomotic obstruction after surgery. T2DM group and RYGB group were lower than NC group in postoperative body weight（P 〈 0. 05）;RYGB group was lower than T2DM group in body weight after surgery（P 〈 0. 05）. NC group had higher body weight after surgery than that before surgery（P 〈 0. 05）;RYGB group had lower body weight after surgery than that＆amp;nbsp;before surgery（P 〈 0. 05）. T2DM group was higher in TNF-α level and lower in adiponectin concentration than NC group before and after surgery（P 〈 0. 05）. RYGB group was higher in TNF-α level and lower in adiponectin concentration than NC group before surgery（P 〈 0. 05）;RYGB group was lower in TNF-α level and higher in adiponectin concentration than T2DM group after surgery（P 〈 0. 05）. RYGB group had lower TNF-α le

关 键 词：糖尿病 2型 胃旁路术 P选择素 脂联素 

分 类 号：R587.1[医药卫生—内分泌]