驯鹿产气荚膜梭菌的分离鉴定  

Isolation and Identification of Clostridium perfringens from a Reindeer

作　　者：韩一啸 周伟[2] 贾敏[2] 祝令伟[2] 纪雪[2] 孙洋[2] 刘军[2] 王海军 郭学军[2] 

机构地区：[1]吉林农业大学动物科技学院,长春130118 [2]军事医学科学院军事兽医研究所,吉林省人兽共患病预防与控制重点实验室,长春130122 [3]吉林省野生动物救护繁育中心,长春130122

出　　处：《野生动物学报》2016年第2期113-117,共5页CHINESE JOURNAL OF WILDLIFE

基　　金：国家“863”项目(2012AA022006);军队医药卫生课题(13CXZ024)

摘　　要：某动物园驯鹿死亡突然,根据其尸体外观变化和剖检病变观察,怀疑为产气荚膜梭菌感染,为进一步确诊,进行了病原分离培养及鉴定试验。在猝死驯鹿的肝、脾、肾、血等样本中分离获得1株革兰氏阳性杆菌(编号XL-1)。经细菌培养特性鉴定、菌落形态观察、分子生物学鉴定,确定分离菌为A型产气荚膜梭菌。药敏实验显示,XL-1对头孢替坦、头孢他啶、氨曲南等药物敏感。A farmed reindeer died suddenly.According to its body appearance and autopsy lesions,we suspected that the reindeer was infected by Clostridum perfringens.In order to further confirmed the causes,the liver,spleen,kidney and blood samples from the dead reindeer were collected.A Clostridium perfringens type A strain was recovered.The strain（XL-1） was then evaluated by cultural characteristics,colony morphology,molecular biology.Drug sensitivity tests showed that XL-1 was sensitive to cefotetan,ceftazidime and aztreonam.

关 键 词：驯鹿 产气荚膜梭菌 病原分离鉴定 

分 类 号：S858.9[农业科学—临床兽医学]