洋葱S-腺苷甲硫氨酸合成酶基因的克隆及分析  

Molecular Cloning and Bioinformatic Analysis of S-adenosylmethionine Synthetase Gene from Allium cepa

作　　者：王晶[1] 刘恩科[2] 王永勤[3] 

机构地区：[1]吕梁学院生命科学系,山西吕梁033001 [2]山西省农业科学院旱地农业研究中心,山西太原030031 [3]北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室,北京100097

出　　处：《山西农业科学》2016年第5期575-578,共4页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31372066);北京市农林科学院科技创新能力建设专项(KJCX201101010)

摘　　要：根据洋葱转录组测序结果设计了S-腺苷甲硫氨酸合成酶基因(Ac SAMS)引物,利用RT-PCR技术和RACE技术克隆了洋葱S-腺苷甲硫氨酸合成酶基因的c DNA全长,命名为Ac SAMS。该c DNA全长1 475 bp,ORF为1 191 bp,编码396个氨基酸的多肽。生物信息学分析表明,Ac SAMS氨基酸序列与紫萼同源性为95%,与短花药野生稻为94%,与桔梗和粳稻为93%。系统发育树结果显示,Ac SAMS与唐菖莆SAMS的亲缘关系最近。该基因的克隆可为进一步研究Ac SAMS在抗旱、抗冻、耐盐等抗逆过程中的作用机理提供理论依据,为开展抗逆基因工程奠定基础。Primers of S-adenosylmethionine synthetase gene were designed based on the result of onion transcriptome sequencing,and full-length c DNA of S-adenosylmethionine synthetase gene in onion was cloned using RT-PCR combined with RACE technology,named Ac SAMS. The total length of c DNA was 1 475 bp and its ORF encoding a polypeptide of 396 amino-acid residues was 1 191 bp.Bioinformatics analysis showed that the homology between the amino acid sequence of Ac SAMS and grimmia was 95%, and the short anthers of wild rice was 94%, and the japonica and platycodon grandiflorum were 93%, respectively. The dendrogram result indicated that the closest genetic relationship existed in Ac SAMS and SAMS Gladiolus grandiflorus. The cloning of the gene lays a foundation for further research on the functional mechanism of Ac SAMS in the process of drought resistance and salt tolerance and the development of genetic improvement.

关 键 词：洋葱 S-腺苷甲硫氨酸合成酶基因 克隆 分析 

分 类 号：S633.2[农业科学—蔬菜学]