IL-1β通过p38、JNK和NF-κB信号通路促进髓核细胞增殖  

IL-1β promotes the proliferation of human nucleus pulposus cells via p38,JNK and NF-κB

作　　者：王小虎[1,2] 吴小涛[1,2] 

机构地区：[1]东南大学医学院,江苏南京210009 [2]东南大学附属中大医院脊柱外科中心,江苏南京210009

出　　处：《东南大学学报（医学版）》2016年第1期32-36,共5页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(81272035);东南大学基础科研扶持项目(3290005431)

摘　　要：目的:探讨白细胞介素1β(IL-1β)对髓核细胞增殖的影响,并深入研究其机制。方法:体外培养人髓核细胞,用IL-1β处理髓核细胞,通过CCK-8实验和Ki67免疫荧光实验检测IL-1β对髓核细胞增殖能力的影响。用Western blotting检测IL-1β对MAPK和NF-κB信号通路的影响。通过对细胞信号通路进行特异性抑制,观察上述通路在IL-1β作用于髓核细胞过程中的作用。结果:CCK-8实验和Ki67免疫荧光实验均提示IL-1β提高髓核细胞的增殖能力。Western blotting实验显示IL-1β显著激活髓核细胞中的MAPK和NF-κB信号通路。使用p38、JNK和NF-κB的抑制剂均能明显抑制IL-1β诱导的髓核细胞增殖,而ERK的抑制剂则不能。结论:IL-1β通过p38、JNK和NF-κB信号通路促进髓核细胞增殖。Objective：To investigate the effect of IL-1β on proliferation of human nucleus pulposus（NP） cells.Methods：NP cells were treated with IL-1β.Cell proliferation was determined by CCK-8 analysis and Ki67 immunofluorescence staining.To identify the mechanism by which IL-1β induced proliferation of NP cells,Western blotting was carried out and selective inhibitors of MAPK and NF-κB signaling pathways were used.Results：Treatment with IL-1β increased cell viability （as determined by CCK-8 analysis） and the number of Ki67-positive NP cells. IL-1β activated MAPK and NF-κB signaling pathways in NP cells. Moreover,inhibition of p38,JNK and NF-κB blocked IL-1β-stimulated proliferation of NP cells. Conclusion：The current findings suggest that the effect of IL-1β on intervertebral disc degeneration involves promotion of the proliferation of human NP cells via the p38,JNK and NF-κB pathways.

关 键 词：白细胞介素1Β 髓核细胞 增殖 MAPK NF-κB 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]