祛风宣痹方对支气管哮喘大鼠肺组织内核因子-κB表达的影响  

Effects of Qufeng-xuanbi formula on nuclear facter-κB expression in lung tissue of asthmatic rats

作　　者：史锁芳[1] 李萍[2] 杨兴嫕[2] 

机构地区：[1]江苏省中医院呼吸科,江苏南京210029 [2]南京中医药大学

出　　处：《河北中医》2016年第4期559-561,F0003,共4页Hebei Journal of Traditional Chinese Medicine

基　　金：江苏省“六大人才高峰”第六批高层次人才资助项目[编号:苏人社(R)通(2009)182号-15];江苏省中医药领军人才项目(编号:J200909)

摘　　要：目的探讨祛风宣痹方治疗支气管哮喘的作用机制。方法将清洁级雄性SD大鼠50只随机分为5组,即空白组、模型组、泼尼松组、祛风宣痹方低剂量组及祛风宣痹方高剂量组,各10只。采用免疫组织化学染色技术,即二氨基联苯胺(DAB)显色检测大鼠肺组织内核因子-κB p65蛋白(NF-κB p65);计算机图像分析技术对肺组织NF-κB p65表达进行定量检测。结果模型组大鼠肺组织构成NF-κB阳性细胞网络,较空白组比较NF-κB细胞密度显著增加(P<0.01);泼尼松组、祛风宣痹方高剂量组肺组织NF-κB细胞密度较模型组显著下降(P<0.01),但祛风宣痹方低剂量组无明显下降(P>0.05)。结论祛风宣痹方有效治疗哮喘的作用与抑制NF-κB活化密切相关。Objective To study the action mechanism of Qufeng- xuanbi formula on the treatment of bronchial asthma. Methods 50 male SD rats（ clean class） were randomly divided into five groups,that blank group,model group,prednisone group,Qufeng- xuanbi formula low dose group（ low dose group） and Qufeng- xuanbi formula high dose group（ high dose group）,10 rats in each group. The immunohistochemical staining method,namely,DAB- staining technique,was used to detect nuclear factor- kappa B P65（ NF- κB p65）. The expression of NF-κB p65 was detected by computer image analysis system. Results All NF- κB p65 staining in model group could be accounted for a net work,and the density of NF- κB p65 staining was higher than that in control group（ P〈0. 01）. The prednisone and high dose group had a significantly lower density of NF- κB p65 staining than that of model group（ P〈0. 01）,but with no obviously decreased as compared with that in low dose group（ P〉0. 05）.Conclusion The therapeutic action of Qufeng- xuanbi formula on bronchial asthma may related to the decrement of activated NF- κB cells.

关 键 词：NF-ΚB 生物合成 肺 疾病模型 动物 哮喘 

分 类 号：R332[医药卫生—人体生理学]